Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/17982
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dc.contributor.authorHuynh, Nhi-
dc.contributor.authorWang, Kai-
dc.contributor.authorYim, Mildred-
dc.contributor.authorDumesny, Chelsea J-
dc.contributor.authorSandrin, Mauro S-
dc.contributor.authorBaldwin, Graham S-
dc.contributor.authorNikfarjam, Mehrdad-
dc.contributor.authorHe, Hong-
dc.date2017-06-19-
dc.date.accessioned2018-07-02T03:58:26Z-
dc.date.available2018-07-02T03:58:26Z-
dc.date.issued2017-06-19-
dc.identifier.citationBMC cancer 2017; 17(1): 431-
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/17982-
dc.description.abstractP21-activated kinase 1 (PAK1) stimulates growth and metastasis of colorectal cancer (CRC) through activation of multiple signalling pathways. Up-regulation of CRC stem cell markers by PAK1 also contributes to the resistance of CRC to 5-fluorouracil. The aim of this study was to investigate the effect of PAK1 depletion and inhibition on the immune system and on intestinal tumour formation in APC∆14/+ mice. The PAK1 KO APC∆14/+ mice were generated by cross-breeding of PAK1 KO mice with APC∆14/+ mice. Splenic lymphocytes were analysed by flow cytometry, and immunohistochemical staining. The numbers of intestinal tumours were counted. Blood cells were also counted. Compared to APC+/+ mice, the numbers of both T- and B- lymphocytes were reduced in the spleen of APC∆14/+ mice. Depletion of PAK1 in APC∆14/+ mice increased the numbers of splenic T- and B- lymphocytes and decreased the numbers of intestinal tumours. Treatment of APC∆14/+ mice with PF-3758309, a PAK inhibitor reduced the numbers of intestinal tumours and increased the numbers of blood lymphocytes. Depletion of active PAK1 up-regulates the immune system of APC∆14/+ mice and suppresses intestinal tumour development. These observations suggest an important role for PAK1 in the immune response to tumours.-
dc.language.isoeng-
dc.subjectAPC-
dc.subjectIntestinal tumour-
dc.subjectLymphocytes-
dc.subjectPAK1-
dc.titleDepletion of p21-activated kinase 1 up-regulates the immune system of APC∆14/+ mice and inhibits intestinal tumorigenesis.-
dc.typeJournal Article-
dc.identifier.journaltitleBMC cancer-
dc.identifier.affiliationDepartment of Surgery, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia-
dc.identifier.doi10.1186/s12885-017-3432-0-
dc.identifier.orcid0000-0002-0944-8747-
dc.identifier.orcid0000-0003-4866-276X-
dc.identifier.pubmedid28629331-
dc.type.austinJournal Article-
local.name.researcherHe, Hong
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
crisitem.author.deptSurgery (University of Melbourne)-
crisitem.author.deptSurgery (University of Melbourne)-
crisitem.author.deptSurgery (University of Melbourne)-
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