Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/16222
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dc.contributor.authorCarter, Glen P-
dc.contributor.authorBuultjens, Andrew H-
dc.contributor.authorBallard, Susan A-
dc.contributor.authorBaines, Sarah L-
dc.contributor.authorTomita, Takehiro-
dc.contributor.authorJohnson, Paul D R-
dc.contributor.authorFerguson, John K-
dc.contributor.authorSeemann, Torsten-
dc.contributor.authorStinear, Timothy P-
dc.contributor.authorHowden, Benjamin P-
dc.contributor.authorStrachan, Janet-
dc.date2016-08-15-
dc.date.accessioned2016-09-11T04:33:51Z-
dc.date.available2016-09-11T04:33:51Z-
dc.date.issued2016-12-
dc.identifier.citationJournal of Antimicrobial Chemotherapy 2016; 71(12): 3367-3371en_US
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/16222-
dc.description.abstractBACKGROUND: Enterococcus faecium is a major nosocomial pathogen causing significant morbidity and mortality worldwide. Assessment of E. faecium using MLST to understand the spread of this organism is an important component of hospital infection control measures. Recent studies, however, suggest that MLST might be inadequate for E. faecium surveillance. OBJECTIVES: To use WGS to characterize recently identified vancomycin-resistant E. faecium (VREfm) isolates non-typeable by MLST that appear to be causing a multi-jurisdictional outbreak in Australia. METHODS: Illumina NextSeq and Pacific Biosciences SMRT sequencing platforms were used to determine the genome sequences of 66 non-typeable E. faecium (NTEfm) isolates. Phylogenetic and bioinformatics analyses were subsequently performed using a number of in silico tools. RESULTS: Sixty-six E. faecium isolates were identified by WGS from multiple health jurisdictions in Australia that could not be typed by MLST due to a missing pstS allele. SMRT sequencing and complete genome assembly revealed a large chromosomal rearrangement in representative strain DMG1500801, which likely facilitated the deletion of the pstS region. Phylogenomic analysis of this population suggests that deletion of pstS within E. faecium has arisen independently on at least three occasions. Importantly, the majority of these isolates displayed a vancomycin-resistant genotype. CONCLUSIONS: We have identified NTEfm isolates that appear to be causing a multi-jurisdictional outbreak in Australia. Identification of these isolates has important implications for MLST-based typing activities designed to monitor the spread of VREfm and provides further evidence supporting the use of WGS for hospital surveillance of E. faecium.en_US
dc.titleEmergence of endemic MLST non-typeable vancomycin-resistant Enterococcus faeciumen_US
dc.typeJournal Articleen_US
dc.identifier.journaltitleJournal of Antimicrobial Chemotherapyen_US
dc.identifier.affiliationDoherty Applied Microbial Genomics, Department of Microbiology and Immunology, The University of Melbourne, Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australiaen_US
dc.identifier.affiliationDepartment of Microbiology and Immunology, the University of Melbourne, Melbourne, Victoria, Australiaen_US
dc.identifier.affiliationThe Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australiaen_US
dc.identifier.affiliationDepartment of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australiaen_US
dc.identifier.affiliationHunter New England Health, Pathology North and Universities of Newcastle and New England, John Hunter Hospital, Newcastle, NSW, Australiaen_US
dc.identifier.affiliationVictorian Life Sciences Computation Initiative, Carlton, Victoria, Australiaen_US
dc.identifier.pubmedurihttps://pubmed.ncbi.nlm.nih.gov/27530751en_US
dc.identifier.doi10.1093/jac/dkw314en_US
dc.type.contentTexten_US
dc.type.austinJournal Articleen_US
local.name.researcherHowden, Benjamin P
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
crisitem.author.deptInfectious Diseases-
crisitem.author.deptInfectious Diseases-
crisitem.author.deptMicrobiology-
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