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Title: Hepatic ischemia-reperfusion injury modification during liver surgery in rats: pretreatment with nifedipine or misoprostol.
Austin Authors: Hardy, Kenneth John;Tancheroen, S;Shulkes, Arthur
Affiliation: Department of Surgery, Austin Hospital, Melbourne, Australia
Issue Date: 1-Sep-1995
Publication information: Liver Transplantation and Surgery : Official Publication of the American Association For the Study of Liver Diseases and the International Liver Transplantation Society ; 1(5): 302-10
Abstract: The aim of the study was to determine if pretreatment with misoprostol (a prostaglandin analogue) or nifedipine (a calcium antagonist), know protectants of the whole liver, would ameliorate the ischemia-reperfusion injury (IRI) of resected liver associated with vascular occlusion. Male Wistar rats were allocated to 5 groups (n = 20 each group): sham-operated, liver resection only, liver resection plus pretreatment with 0.1 mg/kg misoprostol, 10 mg/kg, or 2 mg/kg nifedipine during the 3 days before IRI with liver resection. Fifteen percent of the liver was made ischemic by 30-minute continuous vascular occlusion, and the remaining 85% nonischemic liver was resected. The model was designed to have survival of the rats so that liver function could be studied over 3 weeks. Seventeen of 20 control resection rats survived indicating a suitable model for study. The bilirubin level was reduced by 25% on postoperative days 3 through 23 with misoprostol. The serum alanine aminotransferase (ALT) peak was significantly lower on day 1 with misoprostol and high-dose nifedipine (both reduced to half the control resection value). There was a modest but significant reduction of serum alkaline phosphatase (SAP) for low-dose nifedipine on days 1, 2, and 23. Prothrombin had a lower peak and lower values on days 1 through 4 with misoprostol. Liver histological changes were minor, being cytoplasmic vacuolization only, and was slightly more marked in the nifedipine groups. Preoperative misoprostol 0.1 mg/kg and nifedipine 10 mg/kg each ameliorate the IRI associated with liver resection, as measured by liver function tests. Different aspects of liver function were altered by the different agents. These results justify initiating a trial for human liver resections.
Gov't Doc #: 9346587
Journal: Liver transplantation and surgery : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Type: Journal Article
Subjects: Animals
Calcium Channel Blockers.therapeutic use
Disease Models, Animal
Liver.blood supply.drug
Liver Diseases.etiology.physiopathology.prevention & control
Liver Function Tests
Misoprostol.therapeutic use
Nifedipine.therapeutic use
Organ Size
Oxytocics.therapeutic use
Random Allocation
Rats, Wistar
Reperfusion Injury.etiology.physiopathology.prevention & control
Appears in Collections:Journal articles

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