Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13477
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dc.contributor.authorMurone, Carmelen
dc.contributor.authorPerich, R Ben
dc.contributor.authorSchlawe, Ien
dc.contributor.authorMüller-Esterl, Wen
dc.contributor.authorMendelsohn, Frederick AOen
dc.contributor.authorChai, Syn Yen
dc.date.accessioned2015-05-16T03:20:12Z
dc.date.available2015-05-16T03:20:12Z
dc.date.issued1996-06-01en
dc.identifier.citationImmunopharmacology; 33(1-3): 108-12en
dc.identifier.govdoc8856126en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/13477en
dc.description.abstractThe localization and characterization of bradykinin B2 receptors in sheep uterus was carried out using a radiolabelled B2 receptor ligand, 3,4-hydroxyphenypropionyl-D-Arg0-[Hyp3,Thi5,D-++ +Tic7,D-Oic8] bradykinin (HPP-HOE140). Competition of the radioligand, [125I]HPP-HOE140, from membrane preparations of anoestrus sheep uterus by bradykinin agonists and antagonists revealed the presence of high- and low-affinity binding sites with ligand specificity typical of the bradykinin B2 receptor. Using in vitro autoradiography on tissue sections, intense binding was visible over the superficial epithelial layer of the endometrium and inner third of the myometrium of anoestrus sheep uterus. Bradykinin B2 receptors in the myometrium were down regulated in pregnant sheep uterus. We demonstrate that binding studies using [125I]HPP-HOE140 offer high sensitivity and specificity for characterization, quantitation and localization of the bradykinin B2 receptors in tissues and offers new information on uterine bradykinin B2 receptors.en
dc.language.isoenen
dc.subject.otherAnestrus.metabolismen
dc.subject.otherAnimalsen
dc.subject.otherAutoradiographyen
dc.subject.otherBinding, Competitiveen
dc.subject.otherBradykinin.analogs & derivatives.metabolismen
dc.subject.otherEndometrium.metabolismen
dc.subject.otherFemaleen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherMyometrium.metabolismen
dc.subject.otherPregnancyen
dc.subject.otherRadioligand Assayen
dc.subject.otherReceptor, Bradykinin B2en
dc.subject.otherReceptors, Bradykinin.metabolismen
dc.subject.otherSheepen
dc.subject.otherUterus.metabolismen
dc.titleLocalization of bradykinin B2 receptors in sheep uterus.en
dc.typeJournal Articleen
dc.identifier.journaltitleImmunopharmacologyen
dc.identifier.affiliationUniversity of Melbourne, Department of Medicine, Austin, Australiaen
dc.description.pages108-12en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8856126en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
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