Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13409
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dc.contributor.authorGilbert, Richard Een
dc.contributor.authorMcNally, P Gen
dc.contributor.authorCox, Allison Jen
dc.contributor.authorDziadek, Men
dc.contributor.authorRumble, J Ren
dc.contributor.authorCooper, Mark Een
dc.contributor.authorJerums, Georgeen
dc.date.accessioned2015-05-16T03:15:16Z
dc.date.available2015-05-16T03:15:16Z
dc.date.issued1995-10-01en
dc.identifier.citationKidney International; 48(4): 1216-25en
dc.identifier.govdoc8569083en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13409en
dc.description.abstractRenal enlargement is a characteristic feature of diabetes in humans and experimental animals that may predict subsequent renal disease. The biological processes involved in diabetes-related kidney growth are complex and involve changes in extracellular matrix, cell hypertrophy and hyperplasia. Secreted protein acidic and rich in cysteine (SPARC) is an extracellular matrix protein with anti-adhesive, antiproliferative and matrix remodeling properties. We examined kidney SPARC gene expression and protein content in early experimental diabetes. By Northern blot analysis, kidney SPARC mRNA fell in diabetic animals at day 1 to 40 +/- 15% of controls levels (mean +/- SEM, P < 0.01) to 42% +/- 11% on day 3 (P < 0.01) with a further decrease at day 7 to 29 +/- 7% (P < 0.001). In situ hybridization demonstrated SPARC mRNA within glomeruli renal interstitial cells and in blood vessels but not in tubular epithelial cells. SPARC mRNA was decreased in diabetic rats within a change in the pattern of distribution. By immunofluorescence, SPARC protein was detected in glomeruli and tubular basement membrane. Diabetes was associated with a decrease in SPARC protein at both sites. These data demonstrate that the onset of diabetes-related kidney growth is associated with a reduction in SPARC mRNA and protein. In the context of the known biological actions of SPARC, the findings in the present study implicate this matrix protein in the pathogenesis of diabetes related kidney growth.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherDiabetes Mellitus, Experimental.genetics.pathologyen
dc.subject.otherDiabetic Nephropathies.etiology.genetics.pathologyen
dc.subject.otherGene Expressionen
dc.subject.otherGlycoproteins.genetics.metabolismen
dc.subject.otherIn Situ Hybridizationen
dc.subject.otherKidney.metabolism.pathologyen
dc.subject.otherMaleen
dc.subject.otherRNA, Messenger.genetics.metabolismen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.titleSPARC gene expression is reduced in early diabetes-related kidney growth.en
dc.typeJournal Articleen
dc.identifier.journaltitleKidney Internationalen
dc.identifier.affiliationEndocrinology Unit, Austin Hospital, Victoria, Australiaen
dc.description.pages1216-25en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8569083en
dc.type.austinJournal Articleen
local.name.researcherJerums, George
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
crisitem.author.deptEndocrinology-
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