Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12865
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dc.contributor.authorFindlay, D Men
dc.contributor.authorMartin, T Jen
dc.date.accessioned2015-05-16T02:36:50Z
dc.date.available2015-05-16T02:36:50Z
dc.date.issued1986-06-01en
dc.identifier.citationJournal of Bone and Mineral Research : the Official Journal of the American Society For Bone and Mineral Research; 1(3): 277-83en
dc.identifier.govdoc2845727en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/12865en
dc.description.abstractThe fate of 125I-labeled calcitonin and calcitonin receptors in BEN and UMR 106-06 cells was studied after binding of the ligand. At 37 degrees C but not at 4 degrees C, 125I-labeled salmon calcitonin bound to both cell types and was internalized as evidenced by increasing resistance to removal by acid pH. This process was dependent on de novo protein synthesis. Cells were pretreated with salmon calcitonin and washed with acidified buffer to release cell-surface bound hormone and to allow assessment of cell-surface receptor concentration. It was found that initially there was a temperature- and time-dependent calcitonin-induced loss of binding capacity after exposure to salmon calcitonin, suggestive of endocytosis of the calcitonin-receptor complex. In the continued presence of calcitonin, receptors were lost only at the same rate as the normal turnover of calcitonin receptors as assessed in the presence of cycloheximide. These data are consistent with the presence of two functional populations of calcitonin receptors, only one of which is induced to internalize by ligand binding.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherBone Neoplasms.metabolismen
dc.subject.otherCalcitonin.pharmacokineticsen
dc.subject.otherCarcinoma, Squamous Cell.metabolismen
dc.subject.otherCycloheximide.pharmacologyen
dc.subject.otherEndocytosisen
dc.subject.otherHydrogen-Ion Concentrationen
dc.subject.otherLung Neoplasms.metabolismen
dc.subject.otherOsteosarcoma.metabolismen
dc.subject.otherReceptors, Calcitoninen
dc.subject.otherReceptors, Cell Surface.metabolismen
dc.subject.otherSalmonen
dc.subject.otherTemperatureen
dc.subject.otherTumor Cells, Cultureden
dc.titleKinetics of calcitonin receptor internalization in lung cancer (BEN) and osteogenic sarcoma (UMR 106-06) cells.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Bone and Mineral Researchen
dc.identifier.affiliationDepartment of Clinical Pharmacology, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1002/jbmr.5650010306en
dc.description.pages277-83en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/2845727en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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