Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12726
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dc.contributor.authorPereira, Lloyd Aen
dc.contributor.authorHugo, Honor Jen
dc.contributor.authorMalaterre, Jordaneen
dc.contributor.authorHuiling, Xuen
dc.contributor.authorSonza, Secondoen
dc.contributor.authorCures, Alinaen
dc.contributor.authorPurcell, Damian F Jen
dc.contributor.authorRamsland, Paul Aen
dc.contributor.authorGerondakis, Stevenen
dc.contributor.authorGonda, Thomas Jen
dc.contributor.authorRamsay, Robert Gen
dc.date.accessioned2015-05-16T02:27:29Z
dc.date.available2015-05-16T02:27:29Z
dc.date.issued2015-04-08en
dc.identifier.citationPLoS One 2015; 10(4): e0122919en
dc.identifier.govdoc25853889en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/12726en
dc.description.abstractMYB transcriptional elongation is regulated by an attenuator sequence within intron 1 that has been proposed to encode a RNA stem loop (SLR) followed by a polyU tract. We report that NFκBp50 can bind the SLR polyU RNA and promote MYB transcriptional elongation together with NFκBp65. We identified a conserved lysine-rich motif within the Rel homology domain (RHD) of NFκBp50, mutation of which abrogated the interaction of NFκBp50 with the SLR polyU and impaired NFκBp50 mediated MYB elongation. We observed that the TAR RNA-binding region of Tat is homologous to the NFκBp50 RHD lysine-rich motif, a finding consistent with HIV Tat acting as an effector of MYB transcriptional elongation in an SLR dependent manner. Furthermore, we identify the DNA binding activity of NFκBp50 as a key component required for the SLR polyU mediated regulation of MYB. Collectively these results suggest that the MYB SLR polyU provides a platform for proteins to regulate MYB and reveals novel nucleic acid binding properties of NFκBp50 required for MYB regulation.en
dc.language.isoenen
dc.titleMYB Elongation Is Regulated by the Nucleic Acid Binding of NFκB p50 to the Intronic Stem-Loop Region.en
dc.typeJournal Articleen
dc.identifier.journaltitlePLoS Oneen
dc.identifier.affiliationSir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, 3010, Australiaen
dc.identifier.affiliationVictorian Breast Cancer Consortium, Invasion and Metastasis Unit, St Vincent's Institute of Medical Research, Melbourne, Victoria, 3065, Australiaen
dc.identifier.affiliationSchool of Pharmacy University of Queensland, Woolloongabba, Queensland, 4102, Australiaen
dc.identifier.affiliationAustralian Centre for Blood Diseases, Monash University, Prahran, Victoria 3004, Australiaen
dc.identifier.affiliationThe Department of Pathology, The University of Melbourne, Parkville, Victoria, 3010, Australiaen
dc.identifier.affiliationCentre for Immunology, Burnet Institute, Melbourne, Victoria, 3004, Australiaen
dc.identifier.affiliationDepartment of Surgery (Austin Health), The University of Melbourne, Heidelberg, Victoria, 3084, Australiaen
dc.identifier.affiliationDifferentiation and Transcription Laboratory, Peter MacCallum Cancer Centre, Locked Bag #1, Melbourne, Victoria, 8006, Australiaen
dc.identifier.affiliationThe Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, 3010, Australiaen
dc.identifier.affiliationDepartment of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria, 3004, Australiaen
dc.identifier.doi10.1371/journal.pone.0122919en
dc.description.pagese0122919en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/25853889en
dc.type.austinJournal Articleen
local.name.researcherRamsland, Paul A
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptSurgery (University of Melbourne)-
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