Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12475
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dc.contributor.authorLaval, Marieen
dc.contributor.authorBaldwin, Graham Sen
dc.contributor.authorShulkes, Arthuren
dc.contributor.authorMarshall, Kathryn Men
dc.date.accessioned2015-05-16T02:10:41Z
dc.date.available2015-05-16T02:10:41Z
dc.date.issued2014-11-13en
dc.identifier.citationAmerican Journal of Physiology. Gastrointestinal and Liver Physiology 2014; 308(2): G76-84en
dc.identifier.govdoc25394662en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/12475en
dc.description.abstractHypoxia, or a low concentration of O2, is encountered in humans undertaking activities such as mountain climbing and scuba diving and is important pathophysiologically as a limiting factor in tumor growth. Although data on the interplay between hypoxia and gastrins are limited, gastrin expression is upregulated by hypoxia in gastrointestinal cancer cell lines, and gastrins counterbalance hypoxia by stimulating angiogenesis in vitro and in vivo. The aim of this study was to determine if higher concentrations of the gastrin precursor progastrin are protective against hypoxia in vivo. hGAS mice, which overexpress progastrin in the liver, and mice of the corresponding wild-type FVB/N strain were exposed to normoxia or hypoxia. Iron status was assessed by measurement of serum iron parameters, real-time PCR for mRNAs encoding critical iron regulatory proteins, and Perls' stain and atomic absorption spectrometry for tissue iron concentrations. FVB/N mice lost weight at a faster rate and had higher sickness scores than hGAS mice exposed to hypoxia. Serum iron levels were lower in hGAS than FVB/N mice and decreased further when the animals were exposed to hypoxia. The concentration of iron in the liver was strikingly lower in hGAS than FVB/N mice. We conclude that increased circulating concentrations of progastrin provide a physiological advantage against systemic hypoxia in mice, possibly by increasing the availability of iron stores. This is the first report of an association between progastrin overexpression, hypoxia, and iron homeostasis.en
dc.language.isoenen
dc.subject.othergastrinen
dc.subject.otherhypoxiaen
dc.subject.otherironen
dc.subject.otherAnimalsen
dc.subject.otherAnoxia.genetics.metabolismen
dc.subject.otherColon.metabolismen
dc.subject.otherErythropoietin.blooden
dc.subject.otherFemaleen
dc.subject.otherGastrins.metabolismen
dc.subject.otherGene Expression.physiologyen
dc.subject.otherIron.metabolismen
dc.subject.otherLiver.metabolismen
dc.subject.otherMaleen
dc.subject.otherMiceen
dc.subject.otherRNA, Messenger.metabolismen
dc.subject.otherReal-Time Polymerase Chain Reaction.methodsen
dc.titleIncreased gastrin gene expression provides a physiological advantage to mice under hypoxic conditions.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican journal of physiology. Gastrointestinal and liver physiologyen
dc.identifier.affiliationDepartment of Surgery, University of Melbourne, Austin Health, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1152/ajpgi.00344.2014en
dc.description.pagesG76-84en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/25394662en
dc.type.austinJournal Articleen
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.cerifentitytypePublications-
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