Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12062
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dc.contributor.authorRana, Keshaen
dc.contributor.authorClarke, Michele Ven
dc.contributor.authorZajac, Jeffrey Den
dc.contributor.authorDavey, Rachel Aen
dc.contributor.authorMacLean, Helen Een
dc.date.accessioned2015-05-16T01:42:28Z
dc.date.available2015-05-16T01:42:28Z
dc.date.issued2014-01-27en
dc.identifier.citationEndocrine Research 2014; 39(3): 130-5en
dc.identifier.govdoc24467187en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/12062en
dc.description.abstractAndrogens (testosterone and dihydrotestosterone) acting via the androgen receptor (AR) are required for male sexual differentiation, and also regulate the development of many other tissues including muscle, fat and bone. We previously generated an AR(lox) mouse line with exon 3 of the AR gene targeted by loxP sites. The deletion of exon 3 is in-frame, so only the DNA binding-dependent actions of the AR are deleted, but non-DNA binding-dependent actions are retained. This line also contained an antibiotic resistance selection cassette, neomycin (neo) in intron 3, which was also flanked by loxP sites. Hemizygous AR(lox) male mice demonstrated a phenotype of hyperandrogenization, with increased mass of androgen-dependent tissues. We hypothesized that this hyperandrogenization was likely to be due to the presence of the neo cassette. In this study, we have generated an AR(lox) neo-negative mouse line, using the EIIa-cre deleter mouse line to remove the neo cassette. Hemizygous AR(lox) neo-negative male mice have a normal phenotype, with normal body mass and normal mass of androgen-dependent tissues including the testis, seminal vesicles, kidney, spleen, heart and retroperitoneal fat. This neo-negative exon 3-targeted mouse line is the only floxed AR mouse line available to study the DNA binding-dependent actions of the AR in a tissue-specific manner, and is suitable for investigation in all tissues. This study demonstrates the importance of removing the selection cassette, which can potentially alter the phenotype of floxed mouse lines even in the absence of detectable effects on target gene expression.en
dc.language.isoenen
dc.subject.otherCre/loxPen
dc.subject.othergene expressionen
dc.subject.otherknockouten
dc.subject.othermouse modelen
dc.subject.otherselection cassetteen
dc.subject.othertargeteden
dc.subject.otherAnimalsen
dc.subject.otherDNA-Binding Proteins.metabolismen
dc.subject.otherFemaleen
dc.subject.otherGene Deletionen
dc.subject.otherGene Knockout Techniques.methodsen
dc.subject.otherMaleen
dc.subject.otherMice, Inbred C57BLen
dc.subject.otherMice, Transgenicen
dc.subject.otherNeomycin.metabolismen
dc.subject.otherPhenotypeen
dc.subject.otherReceptors, Androgen.genetics.metabolismen
dc.titleNormal phenotype in conditional androgen receptor (AR) exon 3-floxed neomycin-negative male mice.en
dc.typeJournal Articleen
dc.identifier.journaltitleEndocrine researchen
dc.identifier.affiliationDepartment of Medicine, Austin Health, University of Melbourne , Heidelberg, VIC , Australiaen
dc.identifier.doi10.3109/07435800.2013.864303en
dc.description.pages130-5en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/24467187en
dc.type.austinJournal Articleen
local.name.researcherZajac, Jeffrey D
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
crisitem.author.deptEndocrinology-
crisitem.author.deptMedicine (University of Melbourne)-
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