Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11975
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dc.contributor.authorGalea, Laurence A-
dc.contributor.authorNewman, Marsali R-
dc.contributor.authorAu, Rita S C-
dc.contributor.authorLeong, Trishe Y-
dc.contributor.authorWilliams, David S-
dc.date.accessioned2015-05-16T01:36:37Z
dc.date.available2015-05-16T01:36:37Z
dc.date.issued2014-01-01-
dc.identifier.citationPathology; 46(1): 15-20en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/11975en
dc.description.abstractThe aim of this study was to establish a scoring method for ploidy analysis using silver in situ hybridisation (SISH) with a chromosome 17 centromere probe. SISH was performed using the Ventana chromosome 17 centromere probe on sections from formalin fixed, paraffin embedded archival cases of complete hydatidiform moles, partial hydatidiform moles and hydropic products of conception with previously established ploidy status (determined by flow cytometry or karyotyping). In order to determine ploidy status, a scoring method was developed based on both the average number of signals per nucleus (ASN) and the percentage of nuclei with three signals (N3S), enumerated in 50 villous cytotrophoblastic and/or stromal cells. The results of four independent observers were compared individually and collectively with previously established ploidy status. There was a highly statistically significant difference between diploid and triploid gestations for ASN (1.86 ± 0.13 and 2.70 ± 0.16 respectively, Student t-test, p < 0.0001) and for N3S (1.14 ± 1.65 and 71.59 ± 14.25 respectively, Student t-test, p < 0.0001). The sensitivity and specificity of the SISH-based assay was 99.1% and 100% respectively for ASN, and 100% and 100% respectively for N3S. A chromosome 17 centromere probe SISH-based assay can reliably distinguish between diploid and triploid gestations. This test has diagnostic utility in distinguishing partial hydatidiform moles from histological mimics.en
dc.language.isoenen
dc.subject.otherCell Nucleus.geneticsen
dc.subject.otherCentromere.geneticsen
dc.subject.otherChromosomes, Human, Pair 17.genetics.ultrastructureen
dc.subject.otherDNA, Neoplasm.analysis.geneticsen
dc.subject.otherDiploidyen
dc.subject.otherFemaleen
dc.subject.otherHumansen
dc.subject.otherHydatidiform Mole.diagnosis.geneticsen
dc.subject.otherIn Situ Hybridization.methodsen
dc.subject.otherPloidiesen
dc.subject.otherPregnancyen
dc.subject.otherSensitivity and Specificityen
dc.subject.otherSilver.diagnostic useen
dc.subject.otherTriploidyen
dc.subject.otherUterine Neoplasms.diagnosis.geneticsen
dc.titleDevelopment of a silver in situ hybridisation based assay for the determination of ploidy status in molar pregnancy diagnosis.en
dc.typeJournal Articleen
dc.identifier.journaltitlePathologyen
dc.identifier.affiliationDepartment of Pathology, Austin Health Department of Pathology, University of Melbourne 3Ludwig Institute for Cancer Research, Melbourne, Victoria, Australiaen
dc.identifier.doi10.1097/PAT.0000000000000025en
dc.description.pages15-20en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/24300715en
dc.type.contentTexten
dc.type.austinJournal Articleen
local.name.researcherNewman, Marsali R
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptPathology-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
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