Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11297
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dc.contributor.authorRana, Keshaen
dc.contributor.authorFam, Barbara Cen
dc.contributor.authorClarke, Michele Ven
dc.contributor.authorPang, Tammy P Sen
dc.contributor.authorZajac, Jeffrey Den
dc.contributor.authorMacLean, Helen Een
dc.date.accessioned2015-05-16T00:53:11Z
dc.date.available2015-05-16T00:53:11Z
dc.date.issued2011-06-28en
dc.identifier.citationAmerican Journal of Physiology. Endocrinology and Metabolism 2011; 301(5): E767-78en
dc.identifier.govdoc21712531en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/11297en
dc.description.abstractIn men, as testosterone levels decrease, fat mass increases and muscle mass decreases. Increased fat mass in men, in particular central obesity, is a major risk factor for type 2 diabetes, cardiovascular disease, and all-cause mortality. Testosterone treatment has been shown to decrease fat mass and increase fat-free mass. We hypothesize that androgens act directly via the DNA binding-dependent actions of the androgen receptor (AR) to regulate genes controlling fat mass and metabolism. The aim of this study was to determine the effect of a global DNA binding-dependent (DBD) AR knockout (DBD-ARKO) on the metabolic phenotype in male mice by measuring body mass, fat mass, food intake, voluntary physical activity, resting energy expenditure, substrate oxidation rates, serum glucose, insulin, lipid, and hormone levels, and metabolic gene expression levels and second messenger protein levels. DBD-ARKO males have increased adiposity despite a decreased total body mass compared with wild-type (WT) males. DBD-ARKO males showed reduced voluntary activity, decreased food intake, increased serum leptin and adiponectin levels, an altered lipid metabolism gene profile, and increased phosphorylated CREB levels compared with WT males. This study demonstrates that androgens acting via the DNA binding-dependent actions of the AR regulate fat mass and metabolism in males and that the increased adiposity in DBD-ARKO male mice is associated with decreased voluntary activity, hyperleptinemia and hyperadiponectinemia and not with insulin resistance, increased food intake, or decreased resting energy expenditure.en
dc.language.isoenen
dc.subject.otherAdiponectin.blooden
dc.subject.otherAdiposity.geneticsen
dc.subject.otherAnimalsen
dc.subject.otherDNA.metabolismen
dc.subject.otherDNA-Binding Proteins.metabolismen
dc.subject.otherDown-Regulationen
dc.subject.otherEating.genetics.physiologyen
dc.subject.otherInsulin Resistance.genetics.physiologyen
dc.subject.otherLeptin.blooden
dc.subject.otherMaleen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred C57BLen
dc.subject.otherMice, Knockouten
dc.subject.otherMotivation.genetics.physiologyen
dc.subject.otherMotor Activity.geneticsen
dc.subject.otherProtein Interaction Domains and Motifs.geneticsen
dc.subject.otherReceptors, Androgen.chemistry.genetics.metabolismen
dc.subject.otherUp-Regulation.geneticsen
dc.titleIncreased adiposity in DNA binding-dependent androgen receptor knockout male mice associated with decreased voluntary activity and not insulin resistance.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican journal of physiology. Endocrinology and metabolismen
dc.identifier.affiliationDepartment of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia en
dc.identifier.doi10.1152/ajpendo.00584.2010en
dc.description.pagesE767-78en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/21712531en
dc.type.austinJournal Articleen
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptEndocrinology-
crisitem.author.deptMedicine (University of Melbourne)-
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