Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11275
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dc.contributor.authorLin, Wen Xuen
dc.contributor.authorFifis, Theodoraen
dc.contributor.authorMalcontenti-Wilson, Caterinaen
dc.contributor.authorNikfarjam, Mehrdaden
dc.contributor.authorMuralidharan, Vijayaragavanen
dc.contributor.authorNguyen, Linhen
dc.contributor.authorChristophi, Christopheren
dc.date.accessioned2015-05-16T00:51:52Z
dc.date.available2015-05-16T00:51:52Z
dc.date.issued2011-05-29en
dc.identifier.citationJournal of Translational Medicine 2011; 9(): 83en
dc.identifier.govdoc21619693en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/11275en
dc.description.abstractPreliminary experimental studies have suggested that the in situ destruction of tumor tissue by local laser ablation (LA) may also stimulate host immunity against cancer. We investigated local and systemic induction of immune responses after laser ablation in the setting of residual tumor.A murine colorectal cancer (CRC) liver metastasis model was used. Selected tumors of liver CRC bearing mice and livers of mice without tumor induction were treated with LA. Liver and tumor tissues from the ablation sites and from distant sites were collected at various time points following LA and changes in CD3+ T cells and Kupffer cells (F4/80 marker) infiltration and the expression of interferon gamma (IFNγ) were investigated by immunohistochemistry and ELISpot. Base line levels of CD3+ T cells and Kupffer cells were established in untreated mice.The presence of tumor induced significant accumulation of CD3+ T cells and Kupffer cells at the tumor-host interface, within the tumor vascular lakes and increased their baseline concentration within the liver parenchyma. LA of the liver induced accumulation of CD3+ T-cells and Kupffer cells at the site of injury and systemic induction of immune responses as discerned by the presence of IFNγ secreting splenocytes. LA of liver tumors induced significant increase of CD3+ T-cells at site of injury, within normal liver parenchyma, and the tumor-host interface of both ablated and distant tumors. In contrast Kupffer cells only accumulated in ablated tumors and the liver parenchyma but not in distant tumors. IFNγ expression increased significantly in ablated tumors and showed an increasing trend in distant tumors.Laser ablation in addition to local tumor destruction induces local and systemic Th1 type immune responses which may play a significant role in inhibiting tumor recurrence from residual micrometastases or circulating tumor cells.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAntigens, CD3.metabolismen
dc.subject.otherColorectal Neoplasms.pathologyen
dc.subject.otherDisease Models, Animalen
dc.subject.otherImmunity.immunologyen
dc.subject.otherInterferon-gamma.metabolismen
dc.subject.otherKupffer Cells.immunology.pathologyen
dc.subject.otherLaser Therapyen
dc.subject.otherLiver.immunology.pathology.surgeryen
dc.subject.otherLiver Neoplasms.immunology.secondary.surgeryen
dc.subject.otherMaleen
dc.subject.otherMiceen
dc.subject.otherTh1 Cells.immunologyen
dc.subject.otherTime Factorsen
dc.titleInduction of Th1Immune responses following laser ablation in a murine model of colorectal liver metastases.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of translational medicineen
dc.identifier.affiliationDepartment of Surgery, University of Melbourne, Austin Hospital, Heidelberg, Australiaen
dc.identifier.doi10.1186/1479-5876-9-83en
dc.description.pages83en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/21619693en
dc.type.austinJournal Articleen
local.name.researcherChristophi, Christopher
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptSurgery (University of Melbourne)-
crisitem.author.deptSurgery (University of Melbourne)-
crisitem.author.deptHepatopancreatobiliary Surgery-
crisitem.author.deptSurgery-
crisitem.author.deptSurgery-
crisitem.author.deptHepatopancreatobiliary Surgery-
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