Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11249
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLee, Nicole K Len
dc.contributor.authorSkinner, Jarrod P Jen
dc.contributor.authorZajac, Jeffrey Den
dc.contributor.authorMacLean, Helen Een
dc.date.accessioned2015-05-16T00:50:17Z
dc.date.available2015-05-16T00:50:17Z
dc.date.issued2011-04-19en
dc.identifier.citationAmerican Journal of Physiology. Endocrinology and Metabolism 2011; 301(1): E172-9en
dc.identifier.govdoc21505150en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/11249en
dc.description.abstractThe aim of this study is to determine if the Odc1 gene, which encodes ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis, is directly regulated by the androgen receptor (AR) in skeletal muscle myoblasts and if Odc1 regulates myoblast proliferation and differentiation. We previously showed that expression of Odc1 is decreased in muscle from AR knockout male mice. In this study, we show in vivo that Odc1 expression is also decreased >60% in muscle from male muscle-specific AR knockout mice. In normal muscle homeostasis, Odc1 expression is regulated by age and sex, reflecting testosterone levels, as muscle of adult male mice expresses high levels of Odc1 compared with age-matched females and younger males. In vitro, expression of Odc1 is 10- and 1.5-fold higher in proliferating mouse C(2)C(12) and human skeletal muscle myoblasts, respectively, than in differentiated myotubes. Dihydrotestosterone increases Odc1 levels 2.7- and 1.6-fold in skeletal muscle cell myoblasts after 12 and 24 h of treatment, respectively. Inhibition of ODC activity in C(2)C(12) myoblasts by α-difluoromethylornithine decreases myoblast number by 40% and 66% following 48 and 72 h of treatment, respectively. In contrast, overexpression of Odc1 in C(2)C(12) myoblasts results in a 27% increase in cell number vs. control when cells are grown under differentiation conditions for 96 h. This prolonged proliferation is associated with delayed differentiation, with reduced expression of the differentiation markers myogenin and Myf6 in Odc1-overexpressing cells. In conclusion, androgens act via the AR to upregulate Odc1 in skeletal muscle myoblasts, and Odc1 promotes myoblast proliferation and delays differentiation.en
dc.language.isoenen
dc.subject.otherAndrogens.pharmacologyen
dc.subject.otherAnimalsen
dc.subject.otherCell Differentiation.drug effectsen
dc.subject.otherCell Proliferation.drug effectsen
dc.subject.otherCells, Cultureden
dc.subject.otherEmbryo, Mammalianen
dc.subject.otherFemaleen
dc.subject.otherGene Expression Regulation, Enzymologic.drug effectsen
dc.subject.otherHumansen
dc.subject.otherMaleen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred C57BLen
dc.subject.otherMice, Transgenicen
dc.subject.otherMuscle, Skeletal.drug effects.metabolism.physiologyen
dc.subject.otherMyoblasts, Skeletal.drug effects.metabolism.physiologyen
dc.subject.otherOrnithine Decarboxylase.genetics.metabolismen
dc.subject.otherPregnancyen
dc.subject.otherReceptors, Androgen.metabolism.physiologyen
dc.subject.otherUp-Regulation.drug effectsen
dc.titleOrnithine decarboxylase is upregulated by the androgen receptor in skeletal muscle and regulates myoblast proliferation.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican journal of physiology. Endocrinology and metabolismen
dc.identifier.affiliationDepartment of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1152/ajpendo.00094.2011en
dc.description.pagesE172-9en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/21505150en
dc.type.austinJournal Articleen
local.name.researcherZajac, Jeffrey D
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptEndocrinology-
crisitem.author.deptMedicine (University of Melbourne)-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

16
checked on Mar 28, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.