Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10853
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dc.contributor.authorHurley, J Cen
dc.contributor.authorTosolini, F Aen
dc.contributor.authorLouis, William Jen
dc.date.accessioned2015-05-16T00:25:53Z
dc.date.available2015-05-16T00:25:53Z
dc.date.issued1991-10-01en
dc.identifier.citationJournal of Clinical Pathology; 44(10): 849-54en
dc.identifier.govdoc1960219en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/10853en
dc.description.abstractThe effects of plasma and chromogenic substrate on the kinetics of the endotoxin-activated Limulus amoebocyte lysate (LAL) assay were determined. A linear correlation was observed between the rate of development of turbidity (optical density 405) with the LAL reagent and the concentration of endotoxin over a four log ten-fold range. Like chromogenic substrate, the addition of dilution and heat treated plasma to the reaction resulted in an increase in optical density proportional to the concentration of plasma present. The presence of the treated plasma also resulted in an accelerated increase in optical density with comparable results when testing plasma at different concentrations and, additionally, serum. This accelerated increase in optical density may not be recognised in assays that monitor the progress of the reaction at a single time point and may confound assays of plasma samples that use chromogenic substrate. Plasma obtained from endotoxin sensitive and resistant strains of mice showed similar effects. The use of kinetic methodology means that a quantitative assay for endotoxin in plasma can be achieved, its variability comparable with that seen with semiquantitative serial dilution but with greater economy of the LAL reagent.en
dc.language.isoenen
dc.subject.otherChromogenic Compoundsen
dc.subject.otherEndotoxins.analysisen
dc.subject.otherHumansen
dc.subject.otherKineticsen
dc.subject.otherLimulus Testen
dc.subject.otherPlasmaen
dc.subject.otherReproducibility of Resultsen
dc.titleQuantitative Limulus lysate assay for endotoxin and the effect of plasma.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of clinical pathologyen
dc.identifier.affiliationDepartment of Clinical Pharmacology and Therapeutics, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages849-54en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/1960219en
dc.type.austinJournal Articleen
local.name.researcherLouis, William J
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
crisitem.author.deptClinical Pharmacology and Therapeutics-
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