Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10731
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dc.contributor.authorChen, Yen
dc.contributor.authorLee, Nicole K Len
dc.contributor.authorZajac, J Den
dc.contributor.authorMacLean, Helen Een
dc.date.accessioned2015-05-16T00:16:30Z
dc.date.available2015-05-16T00:16:30Z
dc.date.issued2008-10-01en
dc.identifier.citationJournal of Endocrinological Investigation; 31(10): 910-8en
dc.identifier.govdoc19092298en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/10731en
dc.description.abstractAndrogens have anabolic actions in skeletal muscle and could potentially act to: (a) increase proliferation of myoblasts; (b) delay differentiation to myotubes; and (c) induce protein accretion in post-proliferative myofibers. To identify the site of androgens action, we investigated the proliferative response of the C2C12 mouse myoblast cell line to testosterone and dihydrotestosterone (DHT) treatment. Neither androgens affected cell proliferation after up to 7 days treatment, nor was there a synergistic effect of androgens on the proliferative response of C2C12 cells to IGF-I treatment. However, proliferating C2C12 cells expressed 0.1% of the level of androgen receptor (AR) mRNA found in adult mouse gastrocnemius muscle (p<0.01). Therefore, we generated mouse C2C12 myoblast cell lines stably transfected with the mouse AR cDNA driven by the SV40 promoter (C2C12-AR). C2C12-AR cell proliferation, differentiation, and protein content were analyzed in response to androgen treatment. Our data demonstrated that androgen treatment does not alter either proliferation rate or differentiation rate of C2C12-AR cells. However, treatment of differentiated C2C12-AR myotubes with 100 nM DHT for 3 days caused a 20% increase in total protein content vs vehicle treatment (p<0.05). This effect was not observed in control C2C12 cells transfected with empty vector. These data suggest that androgens act via the AR to upregulate myotube protein content. This model cell line will be useful to further investigate the molecular mechanisms via which androgens regulate protein accretion.en
dc.language.isoenen
dc.subject.otherAndrogens.pharmacologyen
dc.subject.otherAnimalsen
dc.subject.otherCell Differentiation.drug effectsen
dc.subject.otherCell Lineen
dc.subject.otherCell Proliferation.drug effectsen
dc.subject.otherDihydrotestosterone.pharmacologyen
dc.subject.otherHumansen
dc.subject.otherMiceen
dc.subject.otherMuscle Fibers, Skeletal.metabolismen
dc.subject.otherMuscle, Skeletal.drug effects.growth & developmenten
dc.subject.otherMyoblasts.cytology.drug effectsen
dc.subject.otherReceptors, Androgen.physiologyen
dc.subject.otherTestosterone.pharmacologyen
dc.titleGeneration and analysis of an androgen-responsive myoblast cell line indicates that androgens regulate myotube protein accretion.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of endocrinological investigationen
dc.identifier.affiliationDepartment of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia en
dc.identifier.doi10.1007/BF03346441en
dc.description.pages910-8en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/19092298en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.openairetypeJournal Article-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
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