Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10167
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dc.contributor.authorMulley, William Ren
dc.contributor.authorWee, Janet Len
dc.contributor.authorChristiansen, Daleen
dc.contributor.authorMilland, Julieen
dc.contributor.authorIerino, Francesco Len
dc.contributor.authorSandrin, Mauro Sen
dc.date.accessioned2015-05-15T23:31:56Z
dc.date.available2015-05-15T23:31:56Z
dc.date.issued2006-05-01en
dc.identifier.citationXenotransplantation; 13(3): 248-52en
dc.identifier.govdoc16756567en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/10167en
dc.description.abstractCo-stimulatory blockade is known to inhibit lymphocyte responses and to prolong allograft and xenograft survival. The present study examines the effect of transgenic expression of cytotoxic T lymphocyte-associated molecule-4 immunoglobulin (CTLA4Ig) by a porcine endothelial cell line (PIEC) transduced by a lentiviral vector, on primed xenogeneic T-cell proliferative and cytokine responses.Splenocytes from mice primed with PIEC were used as responder cells in a secondary proliferative assay. CTLA4Ig transduced and wild-type PIEC were used as stimulator cells. Responder cells were assayed for proliferation and cytokine production.Proliferation was profoundly inhibited by CTLA4Ig transduced cells compared with control cells. Cytokine analysis by enzyme linked immunospot demonstrated that production of interferon-gamma, IL4 (interleukin 4) and IL10 was inhibited by CTLA4Ig transduced cells compared with control cells.CTLA4Ig inhibited primed indirect xenogeneic T-cell proliferative and cytokine responses in vitro. Expression of immunomodulatory molecules by xenogeneic tissues has potential therapeutic applications for future xenotransplantation.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAnimals, Genetically Modifieden
dc.subject.otherCell Lineen
dc.subject.otherCytokines.analysisen
dc.subject.otherEndothelium, Vascularen
dc.subject.otherGenetic Vectorsen
dc.subject.otherImmunoconjugates.geneticsen
dc.subject.otherLentivirus.geneticsen
dc.subject.otherLymphocyte Activationen
dc.subject.otherLymphocyte Transfusionen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred BALB Cen
dc.subject.otherSpleen.immunologyen
dc.subject.otherSwineen
dc.subject.otherT-Lymphocytes.immunologyen
dc.subject.otherTransplantation, Heterologous.immunologyen
dc.titleLentiviral expression of CTLA4Ig inhibits primed xenogeneic lymphocyte proliferation and cytokine responses.en
dc.typeJournal Articleen
dc.identifier.journaltitleXenotransplantationen
dc.identifier.affiliationMolecular Immunogenetics Laboratory, The Austin Research Institute, Austin Health, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1111/j.1399-3089.2006.00297.xen
dc.description.pages248-52en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/16756567en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
crisitem.author.deptSurgery (University of Melbourne)-
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