Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9834
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dc.contributor.authorHasin, Yen
dc.contributor.authorKneen, M Men
dc.contributor.authorCraik, D Jen
dc.contributor.authorNayler, W Gen
dc.date.accessioned2015-05-15T23:05:30Z
dc.date.available2015-05-15T23:05:30Z
dc.date.issued1992-02-01en
dc.identifier.citationClinical and Experimental Pharmacology & Physiology; 19(2): 79-87en
dc.identifier.govdoc1555327en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9834en
dc.description.abstract1. The postulate that the composition of solutions used to reperfuse ischaemic hearts may modulate their ability to synthesize high-energy compounds was tested in isolated rat hearts subjected to 30 min normothermic ischaemia and then reperfused with either Krebs'-Henseleit buffer (K-H) for 20 min (control reperfusion, CR), or a 'myocardial protective solution' (MPS) for 5 min, followed by 15 min K-H (modified reperfusion, MR). The 'myocardial protective solution' was designed to protect against damage caused by sodium and calcium accumulation and by free radicals. Metabolic precursors were also included to promote and support adenosine triphosphate (ATP) resynthesis during reperfusion under both aerobic and hypoxic conditions. 2. 31P nuclear magnetic resonance (NMR) was used to measure tissue ATP and creatine phosphate (CP), and atomic absorption spectrometry was used to measure Ca++. Early during CR, ATP recovered to 28% of the pre-ischaemic value, but fell to 5.5% with continued perfusion. Similarly, CP recovered to 45.5% of the pre-ischaemic level during early CR but fell to 25.5% with continued perfusion. 3. Better maintenance of ATP was seen during MR with oxygenated MPS (O2-MR), the final ATP remaining at 16.9% of the pre-ischaemic level. During O2-MR, CP recovered to 43.55 of the pre-ischaemic level but was not maintained and fell to a final level of 29.5%. 4. During MR with O2-free MPS (non-O2-MR), there was no reperfusion-associated fall in ATP or CP, with the levels maintained at 26.6% and 34.55, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)en
dc.language.isoenen
dc.subject.otherAdenosine Triphosphate.biosynthesisen
dc.subject.otherAnimalsen
dc.subject.otherCalcium.metabolismen
dc.subject.otherCoronary Disease.metabolismen
dc.subject.otherFemaleen
dc.subject.otherMaleen
dc.subject.otherMyocardial Reperfusionen
dc.subject.otherMyocardial Reperfusion Injury.prevention & controlen
dc.subject.otherMyocardium.metabolismen
dc.subject.otherPhosphocreatine.metabolismen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred Strainsen
dc.titleRelationship between ATP resynthesis and calcium accumulation in the reperfused rat heart.en
dc.typeJournal Articleen
dc.identifier.journaltitleClinical and Experimental Pharmacology & Physiologyen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages79-87en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/1555327en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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