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Title: | Size-dependent immunogenicity: therapeutic and protective properties of nano-vaccines against tumors. | Austin Authors: | Fifis, Theodora;Gamvrellis, Anita;Crimeen-Irwin, Blessing;Pietersz, Geoffrey A;Li, Jie;Mottram, Patricia L;McKenzie, Ian F C;Plebanski, Magdalena | Affiliation: | Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia | Issue Date: | 1-Sep-2004 | Publication information: | Journal of Immunology (baltimore, Md. : 1950); 173(5): 3148-54 | Abstract: | Infection can protect against subsequent disease by induction of both humoral and cellular immunity, but inert protein-based vaccines are not as effective. In this study, we present a new vaccine design, with Ag covalently conjugated to solid core nano-beads of narrowly defined size (0.04-0.05 microm) that localize to dendritic cells (DEC205(+) CD40(+), CD86(+)) in draining lymph nodes, inducing high levels of IFN-gamma production (CD8 T cells: precursor frequencies 1/5000 to 1/1000) and high Ab titers in mice. Conjugation of Ag to these nano-beads induced responses that were significantly higher (2- to 10-fold) than those elicited by other bead sizes, and higher than a range of currently used adjuvants (alum, QuilA, monophosphoryl lipid A). Responses were comparable to CFA/IFA immunization for Abs and ex vivo peptide-pulsed dendritic cell immunization for CD8 T cells. A single dose of Ag-conjugated beads protected mice from tumors in two different model challenges and caused rapid clearance of established tumors in mice. Thus, a range of Ags conjugated to nano-beads was effective as immunogens in both therapeutic and prophylactic scenarios. | Gov't Doc #: | 15322175 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/9795 | Journal: | Journal of Immunology (Baltimore, Md. : 1950) | URL: | https://pubmed.ncbi.nlm.nih.gov/15322175 | Type: | Journal Article | Subjects: | Adjuvants, Immunologic.pharmacology Animals Antigens.immunology Cancer Vaccines.immunology.pharmacology Disease Models, Animal Mice Nanotechnology Nanotubes Neoplasms.drug therapy.immunology.prevention & control |
Appears in Collections: | Journal articles |
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