Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9789
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dc.contributor.authorPaterson, Adrienne Cen
dc.contributor.authorLockhart, Sharon Men
dc.contributor.authorBaker, Josephineen
dc.contributor.authorNeumann, Gregen
dc.contributor.authorBaldwin, Graham Sen
dc.contributor.authorShulkes, Arthuren
dc.date.accessioned2015-05-15T23:01:38Z
dc.date.available2015-05-15T23:01:38Z
dc.date.issued2004-08-12en
dc.identifier.citationEndocrinology 2004; 145(11): 5129-40en
dc.identifier.govdoc15308616en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9789en
dc.description.abstractAmidated and nonamidated progastrin-derived peptides have distinct biological activities that are mediated by a range of receptor subtypes. The objective was to determine the nature of the stored and secreted progastrin-derived peptides and to investigate whether progastrin release is regulated by gastric acidity. Using an antiserum directed to the C terminus of progastrin for identification and to monitor purification, C-terminal flanking peptides (CTFP) of progastrin (prog(76-83), prog(77-83), and prog(78-83) in approximately equivalent amounts) were isolated and identified from extracts of sheep antrum using ion exchange, HPLC, and mass spectrometry. Only trace amounts of full-length progastrin were present. Progastrin CTFP was the predominant progastrin-derived peptide in the antrum [progastrin CTFP/gastrin amide (Gamide) = 3]. Similarly, progastrin CTFP was the major circulating form in the antral (CTFP, 710 +/- 62 pmol/liter; Gamide, 211 +/- 35 pmol/liter) and jugular (CTFP, 308 +/- 16 pmol/liter; gastrin amide, 32 +/- 3 pmol/liter) veins. Alteration of gastric acidity in sheep by iv infusion of a H/K-adenosine triphosphatase inhibitor or somatostatin or by intragastric infusion of HCl demonstrated that the CTFP concentrations changed, although to a lesser extent than the changes in circulating gastrin amide. We conclude that the CTFP of progastrin is the major stored and circulating species of the gastrin gene, and that it is secreted in a regulated fashion rather than constitutively. Because full-length progastrin is bioactive, but is only a minor antral and secreted form, determination of the biological activity of the C-terminal flanking peptides will be important for a complete understanding of gastrin endocrinology.en
dc.language.isoenen
dc.subject.otherAnesthesiaen
dc.subject.otherAnimalsen
dc.subject.otherAnti-Ulcer Agents.pharmacologyen
dc.subject.otherChromatographyen
dc.subject.otherConsciousnessen
dc.subject.otherGastrins.isolation & purification.metabolism.secretionen
dc.subject.otherMass Spectrometryen
dc.subject.otherOmeprazole.pharmacologyen
dc.subject.otherPeptide Fragments.isolation & purification.metabolism.secretionen
dc.subject.otherProtein Precursors.isolation & purification.metabolism.secretionen
dc.subject.otherPyloric Antrum.metabolism.secretionen
dc.subject.otherSheepen
dc.subject.otherSomatostatin.pharmacologyen
dc.titleIdentity and regulation of stored and secreted progastrin-derived peptides in sheep.en
dc.typeJournal Articleen
dc.identifier.journaltitleEndocrinologyen
dc.identifier.affiliationDepartment of Surgery, University of Melbourne, Austin Health, Heidelberg, Victoria 3084, Australiaen
dc.identifier.doi10.1210/en.2004-0912en
dc.description.pages5129-40en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/15308616en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
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