Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9780
Title: Transgenic mice that express Cre recombinase in osteoclasts.
Austin Authors: Chiu, W S Maria;McManus, J F;Notini, Amanda J;Cassady, A I;Zajac, J D;Davey, Rachel A
Affiliation: Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Issue Date: 1-Jul-2004
Publication information: Genesis (new York, N.y. : 2000); 39(3): 178-85
Abstract: To study the physiological control of osteoclasts, the bone resorbing cells, we generated transgenic mice carrying the Cre recombinase gene driven by either the tartrate-resistant acid phosphatase (TRAP) or cathepsin K (Ctsk) promoters. TRAP-Cre and Ctsk-Cre transgenic mouse lines were characterized by breeding with LacZ ROSA 26 (R26R) reporter mice and immunohistochemistry for Cre recombinase. The Cre transgene was functional in all lines, with Cre-mediated recombination occurring primarily in the long bones, vertebrae, ribs, and calvaria. Histological analyses of the bones demonstrated that functional Cre protein was present in 1) osteoclasts (Ctsk-Cre); 2) osteoclasts, columnar proliferating, and hypertrophic chondrocytes (TRAP-Cre line 4); and 3) round proliferating chondrocytes (TRAP-Cre line 3). In conclusion, we generated transgenic mouse lines that will enable the deletion of floxed target genes in osteoclasts, which will be valuable tools for studying the regulation of osteoclast function.
Gov't Doc #: 15282744
URI: http://ahro.austin.org.au/austinjspui/handle/1/9780
DOI: 10.1002/gene.20041
URL: https://pubmed.ncbi.nlm.nih.gov/15282744
Type: Journal Article
Subjects: Acid Phosphatase.genetics
Animals
Blotting, Northern
Blotting, Southern
Cathepsin K
Cathepsins.genetics
Chondrocytes.metabolism
Crosses, Genetic
DNA Primers
DNA, Complementary
Gene Expression Profiling
Gene Targeting.methods
Immunohistochemistry
Integrases.metabolism
Isoenzymes.genetics
Mice
Mice, Transgenic
Osteoclasts.metabolism
Promoter Regions, Genetic.genetics
Tissue Distribution
Appears in Collections:Journal articles

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