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Title: The humoral immune response to head and neck cancer antigens as defined by the serological analysis of tumor antigens by recombinant cDNA expression cloning.
Austin Authors: Vaughan, Hilary A;St Clair, Fiona;Scanlan, Matthew J;Chen, Yao-Tsen;Maraskovsky, Eugene;Sizeland, Andrew;Old, Lloyd J;Cebon, Jonathan S 
Affiliation: Cancer Vaccine Laboratory, Ludwig Institute for Cancer Research, Austin Health, Studley Road, Heidelberg, 3084, Australia
Issue Date: 16-Jul-2004
Publication information: Cancer Immunity 2004; 4(): 5
Abstract: Learning to identify tumor and tumor-associated antigens in patients with squamous cell carcinoma of the head and neck (HNSCC) may bring about better diagnostic and prognostic evaluations of the disease, innovative therapies based on immunological approaches, and a better understanding of the biology of tumorigenesis. Serological analysis of tumor antigens by recombinant cDNA expression cloning (SEREX) has been used to identify antigens in head and neck cancer to which patients have produced high-titered IgG antibodies. Four cDNA expression libraries have been screened with sera from 6 head and neck cancer patients. Thirty-seven individual gene products were identified. Thirty-one previously characterized proteins and 6 genes coding for molecules that are only partially characterized or novel were isolated. Tissue expression was evaluated by Northern blot analysis, RT-PCR, and in one case, quantitative real-time PCR (qPCR) using Taqman technology. Clone AU-HN-15 encoded a protein highly expressed in HNSCC tissues and cell lines. Tissue adjacent to the tumor had negligible expression. There was low or negligible expression in normal tissues, except for the brain and thymus. AU-HN-15 is identical to KIAA0530; it is an uncharacterized protein previously cloned from brain tissue and has a zinc finger domain. The cDNA encoding this protein has also been isolated in SEREX screens of testicular cancer, breast cancer, and colorectal cancer. Whether AU-HN-15 represents a tumor-antigen target suitable for prognostic or therapeutic purposes is still being analyzed.
Gov't Doc #: 15255672
Journal: Cancer immunity
Type: Journal Article
Subjects: Antibody Formation.immunology
Antigens, Neoplasm.blood.genetics.immunology
Blotting, Northern
Cancer Vaccines.immunology
Carcinoma, Squamous Cell.immunology
Cloning, Molecular
DNA, Complementary.genetics
Head and Neck Neoplasms.blood.genetics.immunology
Peptide Library
Reverse Transcriptase Polymerase Chain Reaction
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