Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9630
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dc.contributor.authorElliott, S Len
dc.contributor.authorMorgan, Denis Jen
dc.contributor.authorSmallwood, R Aen
dc.date.accessioned2015-05-15T22:47:51Z
dc.date.available2015-05-15T22:47:51Z
dc.date.issued1992-11-03en
dc.identifier.citationBiochemical Pharmacology; 44(9): 1887-90en
dc.identifier.govdoc1449541en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9630en
dc.description.abstractLivers from fasted (N = 16) and fed (N = 22) rats were perfused with harmol (50 microM) for an initial 30 min with normal oxygen delivery (6-10 mumol/min/g liver), then for 45 min with perfusate equilibrated with O2/N2 mixtures, which reduced hepatic oxygen delivery to 0.9-6 mumol/min/g liver, and finally for a further 30 min period of normal oxygenation. Seventy per cent of the harmol eliminated was accounted for as the glucuronide conjugate and approximately 5% as the sulphate conjugate. During the hypoxia phase with fed preparations, decreasing oxygenation did not reduce harmol clearance or harmol glucuronide formation clearance until oxygen delivery was less than 2.5 mumol/min/g liver, whereas with fasted preparations this hypoxic threshold was much higher (5 mumol/min/g liver). Below the hypoxic threshold, harmol clearance was linearly related to oxygen delivery in both groups. Hepatic tissue concentrations of unchanged harmol at the end of the hypoxia phase were double those after the same period of normal oxygenation, whereas tissue harmol glucuronide concentrations were similar. By establishing a hypoxic threshold for reduced oxygen availability this study shows that harmol glucuronidation is relatively insensitive to hypoxia, but sensitivity increases markedly in fasted animals.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAnoxia.metabolismen
dc.subject.otherBody Weight.physiologyen
dc.subject.otherFasting.metabolismen
dc.subject.otherGlucuronates.metabolismen
dc.subject.otherHarmine.analogs & derivatives.metabolism.pharmacokineticsen
dc.subject.otherHumansen
dc.subject.otherLiver.metabolismen
dc.subject.otherMaleen
dc.subject.otherOxygen.metabolismen
dc.subject.otherOxygen Consumption.physiologyen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.subject.otherSensitivity and Specificityen
dc.titleFasting increases the sensitivity of hepatic harmol glucuronidation to hypoxia.en
dc.typeJournal Articleen
dc.identifier.journaltitleBiochemical pharmacologyen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Heidelberg Repatriation Hospital, Victoria, Australiaen
dc.description.pages1887-90en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/1449541en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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