Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9590
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dc.contributor.authorGundlach, Andrew Len
dc.contributor.authorKnobe, K Een
dc.date.accessioned2015-05-15T22:44:41Z
dc.date.available2015-05-15T22:44:41Z
dc.date.issued1992-08-01en
dc.identifier.citationJournal of Neurochemistry; 59(2): 758-61en
dc.identifier.govdoc1385830en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9590en
dc.description.abstractThe expression and distribution of mRNA encoding preproatrial natriuretic peptide (ppANP) in rat brain has been investigated by in situ hybridization of two 35S-labeled synthetic DNA oligonucleotides, based on a cDNA clone sequence that encodes rat ppANP. The highest relative concentrations of ppANP mRNA were detected in the medial preoptic hypothalamic nucleus ("anteroventral/third ventricle region") and the medial habenula. Moderate concentrations of ppANP mRNA were observed in the CA1 pyramidal cells of the hippocampus, the endopiriform nucleus, the arcuate nucleus, the zona incerta, and cells of the pontine tegmental and peduculopontine nuclei. Several of these regions, including the habenula and the hypothalamic areas, have previously been reported to contain atrial natriuretic peptide (ANP)-like immunoreactivity, but the expression of ppANP mRNA in CA1 pyramidal cells suggests the occurrence of differential translation of ppANP mRNA into protein product in different brain regions, or the existence of different immunological forms of the peptide. The abundance of ppANP mRNA in brain was relatively low in comparison with that previously reported for many other mRNA species encoding other brain neuropeptides. These results demonstrate that ANP gene expression occurs in discrete neuronal populations of the CNS and that studies of the regulation of this expression should now be possible using quantitative in situ hybridization.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAtrial Natriuretic Factor.geneticsen
dc.subject.otherBase Sequenceen
dc.subject.otherDNA.analysis.geneticsen
dc.subject.otherGene Expression.geneticsen
dc.subject.otherHistocytochemistryen
dc.subject.otherHypothalamus.chemistryen
dc.subject.otherLimbic System.chemistryen
dc.subject.otherMaleen
dc.subject.otherMolecular Sequence Dataen
dc.subject.otherNucleic Acid Hybridizationen
dc.subject.otherOligonucleotide Probesen
dc.subject.otherProtein Precursors.geneticsen
dc.subject.otherRNA, Messenger.analysis.geneticsen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred Strainsen
dc.subject.otherSulfur Radioisotopesen
dc.titleDistribution of preproatrial natriuretic peptide mRNA in rat brain detected by in situ hybridization of DNA oligonucleotides: enrichment in hypothalamic and limbic regions.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of neurochemistryen
dc.identifier.affiliationUniversity of Melbourne, Department of Medicine, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages758-61en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/1385830en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
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