Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9588
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dc.contributor.authorGu, X Hen
dc.contributor.authorOu, R Cen
dc.contributor.authorCasley, David Jen
dc.contributor.authorDaly, Mark Jen
dc.contributor.authorNayler, W Gen
dc.date.accessioned2015-05-15T22:44:32Z
dc.date.available2015-05-15T22:44:32Z
dc.date.issued1992-05-01en
dc.identifier.citationJournal of Cardiovascular Pharmacology; 19(5): 764-9en
dc.identifier.govdoc1381775en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9588en
dc.description.abstractTo establish whether the density, affinity, or selectivity of endothelin-1 (ET-1) binding sites in cardiac ventricular membranes varies with age, membranes were harvested from 5- to 7-day-, 20-day-, and 8- to 9-week-old Sprague Dawley rats and labeled with [125I]ET-1. Selectivity was established by using cold ET-1, ET-2, ET-3, big ET-1, and (+)PN200-110 to inhibit specific binding of [125I]ET-1. Over the age span studied, selectivity and affinity of the [125I]ET-1 binding sites was unchanged, but density (Bmax) decreased from 209.7 +/- 18.4 at 5-7 days to 154.0 +/- 8.9 (p less than 0.02) at 20 days, and to 89.7 +/- 5.2 (p less than 0.01) fmol/mg protein at 8-9 weeks. These age-dependent differences in Bmax were not accompanied by a change in membrane yield and occurred at a time when the specific binding of (+)[3H]PN200-110 increased.en
dc.language.isoenen
dc.subject.otherAging.metabolismen
dc.subject.otherAnimalsen
dc.subject.otherBinding Sitesen
dc.subject.otherBinding, Competitiveen
dc.subject.otherCalcium.antagonists & inhibitorsen
dc.subject.otherDihydropyridines.metabolism.pharmacologyen
dc.subject.otherEndothelin-1en
dc.subject.otherEndothelins.metabolism.pharmacologyen
dc.subject.otherFemaleen
dc.subject.otherHeart Ventricles.metabolismen
dc.subject.otherIsradipineen
dc.subject.otherMembranes.metabolismen
dc.subject.otherMyocardium.metabolismen
dc.subject.otherOuabain.pharmacologyen
dc.subject.otherProtein Precursors.metabolism.pharmacologyen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred Strainsen
dc.subject.otherReceptors, Cell Surface.metabolismen
dc.subject.otherReceptors, Endothelinen
dc.subject.otherSodium-Potassium-Exchanging ATPase.metabolismen
dc.titleEffect of age on endothelin-1 binding sites in rat cardiac ventricular membranes.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Cardiovascular Pharmacologyen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Australiaen
dc.description.pages764-9en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/1381775en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
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