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Title: Inhibition of PAF-induced activation of human platelets by verapamil.
Austin Authors: Smith, I L;Smith, E A
Affiliation: Department of Haematology, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 1-Dec-1992
Publication information: Blood Coagulation & Fibrinolysis : An International Journal in Haemostasis and Thrombosis; 3(6): 759-63
Abstract: Verapamil, an inhibitor of calcium channels, was shown to inhibit PAF-induced platelet activation. In the presence of 50 microM Verapamil both thromboxane (Tx) formation and release of ATP from dense granules induced by 100 nM PAF was completely inhibited. This concentration of Verapamil only produced partial inhibition of PAF-induced aggregation. It also reduced the size of the PAF-induced calcium (Ca2+) transient demonstrated in Fura-2 loaded platelets. In the absence of extracellular Ca2+, following chelation by EGTA, PAF was still able to induce a Ca2+ transient confirming the requirement of both intra and extracellular Ca2+ for PAF-induced platelet activation. 100 microM Verapamil was able to completely abolish the calcium transient induced by low doses of PAF. These results further suggest that Verapamil is able to inhibit PAF-induced platelet activation by mechanisms apart from blocking Ca2+ channels.
Gov't Doc #: 1336985
Type: Journal Article
Subjects: Adenosine Triphosphate.secretion
Blood Platelets.drug effects.secretion
Calcium Channels.drug effects
Platelet Activating Factor.antagonists & inhibitors
Platelet Activation.drug effects
Thromboxane B2.biosynthesis.secretion
Appears in Collections:Journal articles

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