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https://ahro.austin.org.au/austinjspui/handle/1/9536
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DC Field | Value | Language |
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dc.contributor.author | Gallicchio, Marisa A | en |
dc.contributor.author | Kaun, Christoph | en |
dc.contributor.author | Wojta, Johann | en |
dc.contributor.author | Binder, Bernd | en |
dc.contributor.author | Bach, Leon A | en |
dc.date.accessioned | 2015-05-15T22:39:55Z | |
dc.date.available | 2015-05-15T22:39:55Z | |
dc.date.issued | 2003-10-01 | en |
dc.identifier.citation | Journal of Cellular Physiology; 197(1): 131-8 | en |
dc.identifier.govdoc | 12942549 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/9536 | en |
dc.description.abstract | Urokinase-type plasminogen activator (uPA) binds to its receptor, uPAR, on the surface of cancer cells, leading to the formation of plasmin. Rhabdomyosarcoma (RMS) cell lines secrete high levels of insulin-like growth factor II (IGF-II), suggesting autocrine IGFs play a major role in the unregulated growth and metastasis of RMS. In vitro, IGF-II and IGF-I increased migration of RD cells to 124+/-9% (P<0.01) and 131+/-8% (P<0.05) of control, respectively. IGF-II-induced migration was abolished by insulin-like growth factor binding protein-6 (IGFBP-6) (P<0.01), a relatively specific inhibitor of IGF-II, and by plasminogen activator inhibitor type 1 (PAI-1) (P<0.05). Aprotinin, a plasmin inhibitor, and mannosamine, which inhibits the synthesis of glycosylphosphatidylinositol (GPI), thereby preventing anchorage of GPI-linked proteins such as uPAR to the cell membrane, also decreased IGF-II- (P<0.05 for both) but not IGF-I-induced migration. [Arg54,Arg55]IGF-II and [Leu27]IGF-II, which preferentially bind to the IGF-I and IGF-II/mannose-6-phosphate receptors (IGF-II/M6PR), respectively, both induced RD cell migration to 146+/-8% (P<0.01) and 120+/-7% (P<0.05) of control, respectively. An anti-uPAR anti-serum reduced IGF-II- and IGF-I-induced migration (P<0.05 for both). An anti-low density lipoprotein-related protein (LRP) anti-serum reduced IGF-I-induced migration (P<0.05). IGF-I and -II both increased specific 125I-single chain uPA (scuPA) binding to RD cells in a dose-dependent manner (P<0.01). These results suggest involvement of the PA/plasmin system in IGF-induced migration and indicate important roles these systems may have in RMS metastasis. | en |
dc.language.iso | en | en |
dc.subject.other | Aprotinin.metabolism | en |
dc.subject.other | Cell Movement.drug effects.physiology | en |
dc.subject.other | Dose-Response Relationship, Drug | en |
dc.subject.other | Embryo, Mammalian | en |
dc.subject.other | Enzyme-Linked Immunosorbent Assay | en |
dc.subject.other | Hexosamines.metabolism | en |
dc.subject.other | Humans | en |
dc.subject.other | Insulin-Like Growth Factor Binding Protein 6.metabolism | en |
dc.subject.other | Insulin-Like Growth Factor I.metabolism | en |
dc.subject.other | Insulin-Like Growth Factor II.genetics.metabolism | en |
dc.subject.other | Mutation | en |
dc.subject.other | Plasminogen Activator Inhibitor 1.biosynthesis | en |
dc.subject.other | Receptors, Cell Surface.drug effects.metabolism | en |
dc.subject.other | Receptors, Urokinase Plasminogen Activator | en |
dc.subject.other | Rhabdomyosarcoma.metabolism | en |
dc.subject.other | Tissue Plasminogen Activator.biosynthesis.drug effects | en |
dc.subject.other | Tumor Cells, Cultured | en |
dc.subject.other | Urokinase-Type Plasminogen Activator.drug effects.metabolism | en |
dc.title | Urokinase type plasminogen activator receptor is involved in insulin-like growth factor-induced migration of rhabdomyosarcoma cells in vitro. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Journal of cellular physiology | en |
dc.identifier.affiliation | Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre (Austin Campus), Heidelberg, Victoria, Australia | en |
dc.identifier.doi | 10.1002/jcp.10352 | en |
dc.description.pages | 131-8 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/12942549 | en |
dc.type.austin | Journal Article | en |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
Appears in Collections: | Journal articles |
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