Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9535
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dc.contributor.authorTahtis, Kikien
dc.contributor.authorLee, Fook-Theanen
dc.contributor.authorWheatley, Jennifer Men
dc.contributor.authorGarin-Chesa, Pilaren
dc.contributor.authorPark, John Een
dc.contributor.authorSmyth, Fiona Een
dc.contributor.authorObata, Yuichien
dc.contributor.authorStockert, Elisabethen
dc.contributor.authorHall, Cathrine Men
dc.contributor.authorOld, Lloyd Jen
dc.contributor.authorRettig, Wolfgang Jen
dc.contributor.authorScott, Andrew Men
dc.date.accessioned2015-05-15T22:39:50Z
dc.date.available2015-05-15T22:39:50Z
dc.date.issued2003-08-01en
dc.identifier.citationMolecular Cancer Therapeutics; 2(8): 729-37en
dc.identifier.govdoc12939462en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9535en
dc.description.abstractAntigens and receptors that are highly expressed on tumor stromal cells, such as fibroblast activation protein (FAP), are attractive targets for antibody-based therapies because the supporting stroma and vessel network is essential for a solid neoplasm to grow beyond a size of 1-2 mm. The in vivo characterization of antibodies targeting human stromal or vessel antigens is hindered by the lack of an appropriate mouse model system because xenografts in standard mouse models express stromal and vessels elements of murine origin. This limitation may be overcome by the development of a human skin/mouse chimeric model, which is established by transplanting human foreskin on to the lateral flank of severe combined immunodeficient mice. The subsequent inoculation of breast carcinoma MCF-7 cells within the dermis of the transplanted human skin resulted in the production of xenografts expressing stromal and vessel elements of human origin. Widespread expression of human FAP-positive reactive stromal fibroblasts within xenografts was seen up to 2 months posttransplantation and postinjection of cells. Human blood vessel antigen expression also persisted at 2 months posttransplantation and postinjection of cells with murine vessels coexisting with the human vascular supply. The model was subsequently used to evaluate the biodistribution properties of an iodine-131-labeled humanized anti-FAP monoclonal antibody (BIBH-7). The results showed high specific targeting of the stromal compartment of the xenograft, indicating that the model provides a useful and novel approach for the in vivo assessment of the immunotherapeutic potential of molecules targeting human stroma and angiogenic systems.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAntibodies, Monoclonalen
dc.subject.otherAntigens, Neoplasm.analysis.metabolismen
dc.subject.otherBreast Neoplasmsen
dc.subject.otherCell Line, Tumoren
dc.subject.otherGelatinasesen
dc.subject.otherGene Targetingen
dc.subject.otherH-2 Antigens.metabolismen
dc.subject.otherHLA-A Antigens.analysisen
dc.subject.otherHLA-B Antigens.analysisen
dc.subject.otherHLA-C Antigens.analysis.metabolismen
dc.subject.otherHumansen
dc.subject.otherMembrane Proteinsen
dc.subject.otherMiceen
dc.subject.otherMice, SCIDen
dc.subject.otherModels, Animalen
dc.subject.otherNeoplasm Transplantationen
dc.subject.otherSerine Endopeptidases.analysisen
dc.subject.otherSkin.chemistryen
dc.subject.otherSkin Transplantationen
dc.subject.otherStromal Cells.enzymologyen
dc.subject.otherTransplantation, Heterologousen
dc.subject.otherTumor Markers, Biological.analysisen
dc.titleExpression and targeting of human fibroblast activation protein in a human skin/severe combined immunodeficient mouse breast cancer xenograft model.en
dc.typeJournal Articleen
dc.identifier.journaltitleMolecular cancer therapeuticsen
dc.identifier.affiliationTumour Targeting Program, Ludwig Institute for Cancer Research, Melbourne Branch, Austin, Victoria 3084, Australiaen
dc.description.pages729-37en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/12939462en
dc.type.austinJournal Articleen
local.name.researcherScott, Andrew M
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptMolecular Imaging and Therapy-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
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