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https://ahro.austin.org.au/austinjspui/handle/1/9471
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DC Field | Value | Language |
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dc.contributor.author | Larm, Jari A | en |
dc.contributor.author | Shen, Pei-Juan | en |
dc.contributor.author | Gundlach, Andrew L | en |
dc.date.accessioned | 2015-05-15T22:34:43Z | |
dc.date.available | 2015-05-15T22:34:43Z | |
dc.date.issued | 2003-02-01 | en |
dc.identifier.citation | The European Journal of Neuroscience; 17(3): 481-93 | en |
dc.identifier.govdoc | 12581166 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/9471 | en |
dc.description.abstract | Galanin and galanin receptors are widely expressed by neurons in rat brain that either synthesize/release and/or are responsive to, classical transmitters such as gamma-aminobutyric acid, acetylcholine, noradrenaline, histamine, dopamine and serotonin (5-hydroxytryptamine, 5-HT). The dorsal raphé nucleus (DRN) contains approximately 50% of the 5-HT neurons in the rat brain and a high percentage of these cells coexpress galanin and are responsive to exogenous galanin in vitro. However, the precise identity of the galanin receptor(s) present on these 5-HT neurons has not been previously established. Thus, the current study used a polyclonal antibody for the galanin receptor-1 (GalR1) to examine the possible expression of this receptor within the DRN of the rat and for comparative purposes also in the mouse. In the rat, intense GalR1-immunoreactivity (IR) was detected in a substantial population of 5-HT-immunoreactive neurons in the DRN, with prominent receptor immunostaining associated with soma and proximal dendrites. GalR1-IR was also observed in many cells within the adjacent median raphé nucleus. In mouse DRN, neurons exhibited similar levels and distribution of 5-HT-IR to that in the rat, but GalR1-IR was undetectable. Consistent with this, galanin and GalR1 mRNA were also undetectable in mouse DRN by in situ hybridization histochemistry, despite the detection of GalR1 mRNA (and GalR1-IR) in adjacent cells in the periaqueductal grey and other midbrain areas. 5-HT neuron activity in the DRN is primarily regulated via 5-HT1A autoreceptors, via inhibition of adenylate cyclase and activation of inward-rectifying K+ channels. Notably, the GalR1 receptor subtype signals via identical mechanisms and our findings establish that galanin modulates 5-HT neuron activity in the DRN of the rat via GalR1 (auto)receptors. However, these studies also identify important species differences in the relationship between midbrain galanin and 5-HT systems, which should prompt further investigations in relation to comparative human neurochemistry and which have implications for studies of animal models of relevant neurological conditions such as stress, anxiety and depression. | en |
dc.language.iso | en | en |
dc.subject.other | Animals | en |
dc.subject.other | Autoreceptors.drug effects | en |
dc.subject.other | Fluorescent Antibody Technique | en |
dc.subject.other | Galanin.biosynthesis | en |
dc.subject.other | Image Processing, Computer-Assisted | en |
dc.subject.other | Immunohistochemistry | en |
dc.subject.other | In Situ Hybridization | en |
dc.subject.other | Male | en |
dc.subject.other | Mice | en |
dc.subject.other | Mice, Inbred C57BL | en |
dc.subject.other | Microscopy, Confocal | en |
dc.subject.other | Neurons.metabolism | en |
dc.subject.other | Peroxidases.metabolism | en |
dc.subject.other | Raphe Nuclei.cytology.drug effects.metabolism | en |
dc.subject.other | Rats | en |
dc.subject.other | Rats, Sprague-Dawley | en |
dc.subject.other | Receptors, Galanin | en |
dc.subject.other | Receptors, Neuropeptide.biosynthesis | en |
dc.subject.other | Serotonin.physiology | en |
dc.subject.other | Species Specificity | en |
dc.subject.other | Synaptic Transmission.physiology | en |
dc.title | Differential galanin receptor-1 and galanin expression by 5-HT neurons in dorsal raphé nucleus of rat and mouse: evidence for species-dependent modulation of serotonin transmission. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | The European journal of neuroscience | en |
dc.identifier.affiliation | Howard Florey Institute of Experimental Physiology and Medicine, Austin & Repatriation Medical Centre, The University of Melbourne, Victoria, Australia | en |
dc.description.pages | 481-93 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/12581166 | en |
dc.type.austin | Journal Article | en |
item.openairetype | Journal Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
Appears in Collections: | Journal articles |
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