Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9462
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dc.contributor.authorMcQueen, Kimen
dc.contributor.authorKovac, Suzanaen
dc.contributor.authorHo, Po-Kien
dc.contributor.authorRorison, Kristyen
dc.contributor.authorPannequin, Julieen
dc.contributor.authorNeumann, Gregen
dc.contributor.authorShulkes, Arthuren
dc.contributor.authorBaldwin, Graham Sen
dc.date.accessioned2015-05-15T22:34:01Z
dc.date.available2015-05-15T22:34:01Z
dc.date.issued2002-10-01en
dc.identifier.citationJournal of Protein Chemistry; 21(7): 465-71en
dc.identifier.govdoc12523650en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9462en
dc.description.abstractThe bacterial expression of human progastrin(6-80) has been reported previously [Baldwin, G.S. et al. (2001) J. Biol. Chem. 276: 7791-7796]. The aims of the present study were to prepare full-length recombinant human progastrin(1-80) and to compare its biological activity with that of progastrin(6-80) in vitro, to determine whether or not the N-terminal five amino acids contributed to activity. A fusion protein of glutathione-S-transferase and human progastrin(1-80) was expressed in Escherichia coli, collected on glutathione-agarose beads, and cleaved with enterokinase. Progastrin(1-80) was purified by reversed-phase and anion exchange HPLC and characterized by radioimmunoassay, amino acid sequencing, and mass spectrometry. No differences were detected in the extent of stimulation by progastrin(1-80) and progastrin(6-80) in proliferation and migration assays with the mouse gastric cell line IMGE-5. We conclude that residues 1-5 of progastrin(1-80) are not essential for biological activity.en
dc.language.isoenen
dc.subject.otherAmino Acid Sequenceen
dc.subject.otherAmino Acids.analysisen
dc.subject.otherAnimalsen
dc.subject.otherCell Division.drug effectsen
dc.subject.otherCell Line, Transformeden
dc.subject.otherCell Movement.drug effectsen
dc.subject.otherChromatography, High Pressure Liquid.methodsen
dc.subject.otherEscherichia coli.genetics.metabolismen
dc.subject.otherGastric Mucosa.cytologyen
dc.subject.otherGastrins.biosynthesis.genetics.pharmacologyen
dc.subject.otherGlutathione Transferase.geneticsen
dc.subject.otherHumansen
dc.subject.otherMiceen
dc.subject.otherMice, Transgenicen
dc.subject.otherMolecular Sequence Dataen
dc.subject.otherProtein Precursors.biosynthesis.genetics.pharmacologyen
dc.subject.otherRadioimmunoassayen
dc.subject.otherRecombinant Fusion Proteins.biosynthesis.genetics.pharmacologyen
dc.subject.otherSpectrometry, Mass, Electrospray Ionizationen
dc.titlePreparation of biologically active recombinant human progastrin(1-80).en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of protein chemistryen
dc.identifier.affiliationUniversity of Melbourne Department of Surgery, Austin and Repatriation Medical Centre, Melbourne, Victoria, Australiaen
dc.description.pages465-71en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/12523650en
dc.type.austinJournal Articleen
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
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