Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9444
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dc.contributor.authorWellard, R Men
dc.contributor.authorAdam, W Ren
dc.date.accessioned2015-05-15T22:32:34Z
dc.date.available2015-05-15T22:32:34Z
dc.date.issued2002-11-01en
dc.identifier.citationMagnetic Resonance in Medicine; 48(5): 810-8en
dc.identifier.govdoc12417995en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9444en
dc.description.abstractThis study utilized the large intrinsic chemical shift range of (133)Cs, a potassium congener, in an NMR study of intracellular cation distribution. It demonstrates two distinct intracellular environments in isolated perfused hepatocytes from cesium-fed rats, evident as compartments with different (133)Cs chemical shifts and containing different proportions of total detected cesium. The chemical shifts of the two intracellular compartments were 2.44 +/- 0.07 and 1.21 +/- 0.18 ppm, relative to the cesium signal from the perfusate. The observation of two distinct intracellular cesium signals suggests slow exchange on an NMR chemical shift time-scale (k exchange > 0.02 s). The area of the high-frequency component represented 62 +/- 10% (N = 12) of the total intracellular cesium signal. Manipulation of the intracellular environment using anoxia with aglycemia or digitonin produced changes in the distribution between the two intracellular compartments, showing their dynamic nature. Changes measured in association with metabolic manipulation suggest cytoplasm and mitochondria as the origin of the high and low-frequency intracellular peaks, respectively.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherCesium.pharmacokineticsen
dc.subject.otherCesium Isotopes.diagnostic useen
dc.subject.otherHepatocytes.metabolismen
dc.subject.otherMagnetic Resonance Spectroscopyen
dc.subject.otherPotassium.metabolismen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.titleFunctional hepatocyte cation compartmentation demonstrated with 133Cs NMR.en
dc.typeJournal Articleen
dc.identifier.journaltitleMagnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicineen
dc.identifier.affiliationBrain Research Institute, Austin and Repatriation Medical Center, Repatriation Campus, Heidelberg West, Australiaen
dc.identifier.doi10.1002/mrm.10287en
dc.description.pages810-8en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/12417995en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
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