Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9282
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKolivas, Sen
dc.contributor.authorDow, Ren
dc.contributor.authorJie, Ren
dc.contributor.authorBaldwin, Graham Sen
dc.contributor.authorShulkes, Arthuren
dc.date.accessioned2015-05-15T22:18:49Z
dc.date.available2015-05-15T22:18:49Z
dc.date.issued2000-11-01en
dc.identifier.citationJournal of Gastroenterology and Hepatology; 15(11): 1257-66en
dc.identifier.govdoc11129218en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9282en
dc.description.abstractGastric acid secretion is stimulated by the action of gastrin, histamine and acetylcholine on their respective receptors. To determine the regulation of synthesis of these receptors during different gastric secretory states a competitive RT-PCR method for quantitating the mRNA for these receptors was developed.Partial cDNA clones (400-500 base pairs (bp)) for the ovine gastrin, histamine (H2) and acetylcholine (M3) receptors were isolated and sequenced. These cDNA constructs were modified by the inclusion of approximately 100 bp of unrelated sequence within the plasmids. cDNA was synthesized from a mixture of known amounts of RNA transcribed from the modified plasmids and from total RNA extracted from sheep stomach. Proportional coamplification of mixed cDNA was demonstrated using common primer sets.All three receptors were more highly expressed in the fundus than the antrum. The concentration of cholecystokinin-B/gastrin receptor mRNA was 75-fold higher in the fundus than in the antrum, and the concentration of both histamine and acetylcholine receptor mRNA were fivefold higher in the fundus than in the antrum. Infusion of gastrin caused a significant increase in fundic histamine mRNA receptor expression, but not in the expression of the gastrin or muscarinic receptors.No significant differences were observed in the levels of receptor mRNA between normal adult and fetal animals despite markedly reduced gastric secretion in the fetus, suggesting that gastric receptor gene expression is not the rate-limiting factor in determining gastric acid secretion in the neonatal animal.en
dc.language.isoenen
dc.subject.otherAgingen
dc.subject.otherAnimalsen
dc.subject.otherAnimals, Newbornen
dc.subject.otherGastric Acid.secretionen
dc.subject.otherGastric Fundus.metabolismen
dc.subject.otherPyloric Antrum.metabolismen
dc.subject.otherRNA, Messenger.analysisen
dc.subject.otherReceptors, Cholecystokinin.chemistryen
dc.subject.otherReceptors, Histamine.chemistryen
dc.subject.otherReceptors, Muscarinic.chemistryen
dc.subject.otherReverse Transcriptase Polymerase Chain Reactionen
dc.subject.otherSheepen
dc.titleQuantitative measurement of mRNA coding for the receptors controlling acid secretion in the ovine fundus and antrum by using RT-PCR.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Gastroenterology and Hepatologyen
dc.identifier.affiliationDepartment of Surgery, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australiaen
dc.description.pages1257-66en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/11129218en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

12
checked on Mar 28, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.