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Title: Chiral determination of mirtazapine in human blood plasma by high-performance liquid chromatography.
Austin Authors: Dodd, Seetal;Burrows, Graham D;Norman, Trevor R 
Affiliation: Department of Psychiatry, University of Melbourne, Austin & Repatriation Medical Centre, Heidelberg, Victoria, Australia
Issue Date: 10-Oct-2000
Publication information: Journal of Chromatography. B, Biomedical Sciences and Applications; 748(2): 439-43
Abstract: A method is described for the determination of the two enantiomers of mirtazapine in human blood plasma by high-performance liquid chromatography. Measurements were performed on drug free plasma spiked with mirtazapine and used to prepare and validate standard curves. Levels of enantiomers of mirtazapine were also measured in patients being treated for depression with racemic mirtazapine. Mirtazapine was separated from plasma by solid-phase extraction using CERTIFY columns. Chromatographic separation was achieved using a Chiralpak AD column and pre-column and compounds were detected by their absorption at 290 nm. Imipramine was used as an internal standard. The assay was validated for each analyte in the concentration range 10-100 ng/ml. The coefficient of variance was 16% and 5.5% for(+)-mirtazapine for 10 and 100 ng/ml control specimens respectively and 15% and 7.3% for mirtazapine for 10 and 100 ng/ml control specimens respectively. This assay is appropriate for use in the clinical range. The range of plasma mirtazapine concentrations from eleven patients taking daily doses of 30-45 mg of racemate was <5 to 69 ng/ml for (+)-mirtazapine and 13-88 ng/ml for (-)-mirtazapine for blood specimens collected 10-17.5 h after taking the dose.
Gov't Doc #: 11087086
Type: Journal Article
Subjects: Antidepressive Agents.blood
Chromatography, High Pressure Liquid.methods
Mianserin.analogs & derivatives.blood
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
Appears in Collections:Journal articles

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