Please use this identifier to cite or link to this item:
https://ahro.austin.org.au/austinjspui/handle/1/9161
Title: | Differential dissociation kinetics explain the binding preference of insulin-like growth factor binding protein-6 for insulin-like growth factor-II over insulin-like growth factor-I. | Austin Authors: | Marinaro, J A;Jamieson, Gary P;Hogarth, P Mark;Bach, Leon A | Affiliation: | University of Melbourne, Department of Medicine, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia | Issue Date: | 7-May-1999 | Publication information: | Febs Letters; 450(3): 240-4 | Abstract: | Insulin-like growth factor binding protein-6 binds insulin-like growth factor-II with a marked preferential affinity over insulin-like growth factor-I. The kinetic basis of this binding preference was studied using surface plasmon resonance. Binding of insulin-like growth factor-I and insulin-like growth factor-II to immobilized insulin-like growth factor binding protein-6 fitted a two-site binding kinetic model. Insulin-like growth factor-I and insulin-like growth factor-II association rates were similar whereas the dissociation rate was approximately 60-fold lower for insulin-like growth factor-II, resulting in a higher equilibrium binding affinity for insulin-like growth factor-II. The equilibrium binding affinities of a series of insulin-like growth factor-II mutants were also explained by differential dissociation kinetics. O-glycosylation had a small effect on the association kinetics of insulin-like growth factor binding protein-6. The insulin-like growth factor binding properties of insulin-like growth factor binding protein-6 are explained by differential dissociation kinetics. | Gov't Doc #: | 10359082 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/9161 | Journal: | FEBS letters | URL: | https://pubmed.ncbi.nlm.nih.gov/10359082 | Type: | Journal Article | Subjects: | Binding, Competitive Cell Line Glycosylation Humans Insulin-Like Growth Factor Binding Protein 6.metabolism Insulin-Like Growth Factor I.metabolism Insulin-Like Growth Factor II.metabolism Kinetics Recombinant Fusion Proteins.metabolism Solutions Surface Plasmon Resonance |
Appears in Collections: | Journal articles |
Show full item record
Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.