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dc.contributor.authorDean, Rachael Gen
dc.contributor.authorMaric, Cen
dc.contributor.authorAldred, G Pen
dc.contributor.authorCasley, David Jen
dc.contributor.authorZhuo, Jen
dc.contributor.authorHarris, Pen
dc.contributor.authorAlcorn, Den
dc.contributor.authorMendelsohn, Frederick AOen
dc.identifier.citationClinical and Experimental Pharmacology & Physiology; 26(1): 48-55en
dc.description.abstract1. Renomedullary interstitial cells (RMIC), abundant throughout the medulla of the kidney, have been demonstrated to have binding sites for many vasoactive peptides, including atrial natriuretic peptide, endothelin, angiotensin II and bradykinin (BK). These observations would support the hypothesis that interactions between RMIC and vasoactive peptides are important in the regulation of renal function. 2. We aimed to localize the BK B2 receptor binding site to RMIC in vivo and to also demonstrate that these receptors are biologically active in vitro. 3. The present study demonstrates BK B2 binding sites on RMIC of the inner stripe of the outer medulla and the inner medulla of the rat kidney in vivo. 4. We further demonstrate that the BK B2 radioligand [125I]-HPP-Hoe140 specifically bound to rat RMIC in vitro. In addition, reverse transcription-polymerase chain reaction detected the mRNA for the BK B2 receptor subtype in cell extracts. 5. For RMIC in vitro, cAMP levels were increased at 1 min and cGMP levels were increased at 2 min after treatment with 10(-10) and 10(-7) mol/L BK, respectively. Inositol 1,4,5-trisphosphate was increased at 10 s treatment with both 10(-6) and 10(-7) mol/L BK. 6. For RMIC in vitro, BK induced an increase in cell proliferation ([3H]-thymidine incorporation) and an increase in extracellular matrix synthesis (ECM; trans-[35S] incorporation), both effects mediated by BK B2 receptors. 7. We conclude that BK B2 receptors are present on RMIC both in vivo and in vitro. These receptors are coupled to intracellular second messenger systems and, in vitro, their stimulation results in cellular proliferation and synthesis of ECM.en
dc.subject.otherBinding Sitesen
dc.subject.otherBradykinin.analogs & derivatives.metabolism.pharmacologyen
dc.subject.otherBradykinin Receptor Antagonistsen
dc.subject.otherCell Division.drug effects.physiologyen
dc.subject.otherCyclic AMP.metabolismen
dc.subject.otherExtracellular Matrix Proteins.biosynthesisen
dc.subject.otherInositol 1,4,5-Trisphosphate.metabolismen
dc.subject.otherIodine Radioisotopesen
dc.subject.otherKidney Medulla.cytology.metabolism.ultrastructureen
dc.subject.otherRNA, Messenger.metabolismen
dc.subject.otherRats, Sprague-Dawleyen
dc.subject.otherReceptor, Bradykinin B2en
dc.subject.otherReceptors, Bradykinin.metabolism.physiologyen
dc.subject.otherSignal Transduction.drug effects.physiologyen
dc.titleRat renomedullary interstitial cells possess bradykinin B2 receptors in vivo and in vitro.en
dc.typeJournal Articleen
dc.identifier.journaltitleClinical and Experimental Pharmacology & Physiologyen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin, Australiaen
dc.type.austinJournal Articleen
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
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