Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/33278
Title: Expression of NEDD4L and ENaC in Urinary Extracellular Vesicles in Pre-eclampsia.
Austin Authors: Leung, P Y M;Katerelos, M;Choy, S ;Cook, N;Lee, M;Paizis, K;Abboud, A;Manning, J A;Mount, P F;Power, D A
Affiliation: Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Australia.;Department of Nephrology, Austin Health, Heidelberg, Australia.;Kidney Laboratory, Institute for Breathing and Sleep, Heidelberg, Australia.
Nephrology
Institute for Breathing and Sleep
Department of Obstetrics & Gynaecology, Mercy Hospital for Women, Heidelberg, Australia.
Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, Australia.
Issue Date: Dec-2023
Publication information: Hypertension in Pregnancy 2023-12; 42(1)
Abstract: To assess changes in expression of renal epithelial sodium channel (ENaC) and NEDD4L, a ubiquitin ligase, in urinary extracellular vesicles (UEV) of pre-eclamptic women compared to normal pregnant controls. Urine was collected from pre-eclamptic women (PE, n = 20) or during normal pregnancy (NP, n = 20). UEV were separated by differential ultracentrifugation. NEDD4L, α-ENaC and γ-ENaC were identified by immunoblotting. There was no difference in the expression of NEDD4L (p = 0.17) and α-ENaC (p = 0.10). PE subjects showed increased expression of γ-ENaC by 6.9-fold compared to NP (p < 0.0001). ENaC expression is upregulated in UEV of pre-eclamptic subjects but was not associated with changes in NEDD4L.
URI: https://ahro.austin.org.au/austinjspui/handle/1/33278
DOI: 10.1080/10641955.2023.2232029
ORCID: 0000-0002-3736-0777
Journal: Hypertension in Pregnancy
Start page: 2232029
PubMed URL: 37417251
ISSN: 1525-6065
Type: Journal Article
Subjects: NEDD4L
Pre-eclampsia
epithelial sodium channel
ubiquitin ligase
urinary extracellular vesicles
Epithelial Sodium Channels/metabolism
Extracellular Vesicles/metabolism
Pre-Eclampsia/metabolism
Nedd4 Ubiquitin Protein Ligases/genetics
Appears in Collections:Journal articles

Show full item record

Page view(s)

56
checked on Nov 22, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.