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Title: Multiparameter immunohistochemistry analysis of HIV DNA, RNA and immune checkpoints in lymph node tissue.
Austin Authors: Richardson, Zuwena A;Deleage, Claire;Tutuka, Candani S A;Walkiewicz, Marzena;Del Río-Estrada, Perla M;Pascoe, Rachel D;Evans, Vanessa A;Reyesteran, Gustavo;Gonzales, Michael;Roberts-Thomson, Samuel;González-Navarro, Mauricio;Torres-Ruiz, Fernanda;Estes, Jacob D;Lewin, Sharon R;Cameron, Paul U
Affiliation: Pathology Department, The Royal Melbourne Hospital, Melbourne, Australia
Launceston General Hospital, Tasmania, Launceston, Australia
Olivia Newton John Cancer Centre Research Institute
Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia
Department of Infectious Diseases, Alfred Hospital and Monash University, Melbourne, Australia
Victorian Infectious Diseases Service, Royal Melbourne Hospital, Melbourne, Australia
The Peter Doherty Institute for Infection and Immunity, The University of Melbourne and Royal Melbourne Hospital, Melbourne, Australia
La Trobe School of Cancer Medicine, La Trobe University, Melbourne, Australia
Frederick National Laboratories for Cancer Research, MD, Frederick, United States of America
Centro de Investigación en Enfermdades Infecciosas, Instituto Nacional de Enfermedades Respiratoriras, Mexico City, Mexico
Vaccine and Gene Therapy Institute and Oregon National Primate Research Center, Oregon Health Science University, Portland, Oregon, USA
Issue Date: 2022
Date: 2021-12-02
Publication information: Journal of immunological methods 2022; 501: 113198
Abstract: The main barrier to a cure for HIV is the persistence of long-lived and proliferating latently infected CD4+ T-cells despite antiretroviral therapy (ART). Latency is well characterized in multiple CD4+ T-cell subsets, however, the contribution of regulatory T-cells (Tregs) expressing FoxP3 as well as immune checkpoints (ICs) PD-1 and CTLA-4 as targets for productive and latent HIV infection in people living with HIV on suppressive ART is less well defined. We used multiplex detection of HIV DNA and RNA with immunohistochemistry (mIHC) on formalin-fixed paraffin embedded (FFPE) cells to simultaneously detect HIV RNA and DNA and cellular markers. HIV DNA and RNA were detected by in situ hybridization (ISH) (RNA/DNAscope) and IHC was used to detect cellular markers (CD4, PD-1, FoxP3, and CTLA-4) by incorporating the tyramide system amplification (TSA) system. We evaluated latently infected cell lines, a primary cell model of HIV latency and excisional lymph node (LN) biopsies collected from people living with HIV (PLWH) on and off ART. We clearly detected infected cells that coexpressed HIV RNA and DNA (active replication) and DNA only (latently infected cells) in combination with IHC markers in the in vitro infection model as well as LN tissue from PLWH both on and off ART. Combining ISH targeting HIV RNA and DNA with IHC provides a platform to detect and quantify HIV persistence within cells identified by multiple markers in tissue samples from PLWH on ART or to study HIV latency.
DOI: 10.1016/j.jim.2021.113198
Journal: Journal of Immunological Methods
PubMed URL: 34863818
Type: Journal Article
Subjects: HIV latency
Immune checkpoints
In situ hybridization
Lymph nodes
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