Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/26134
Title: Detection and Quantification of Myocardial Fibrosis Using Stain-Free Infrared Spectroscopic Imaging.
Austin Authors: Zimmermann, Eric;Mukherjee, Sudipta S;Falahkheirkhah, Kianoush;Gryka, Mark C;Kajdacsy-Balla, Andre;Hasan, Wohaib;Giraud, George;Tibayan, Fred;Raman, Jai S ;Bhargava, Rohit
Affiliation: Center for Developmental Health, Oregon Health & Science University, Portland
The Department of Surgery, St Vincent's Hospital, University of Melbourne, Fitzroy, Victoria, Australia
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana..
Department of Bioengineering
Department of Electrical and Computer Engineering
Mechanical Science and Engineering
Cancer Center at Illinois
Department of Chemical and Biomolecular Engineering
Department of Pathology, University of Illinois at Chicago, Chicago..
Department of Pathology and Laboratory Medicine, Cedars-Sinai, Los Angeles, California
From the Center for Developmental Health, Oregon Health & Science University, Portland
Surgery (University of Melbourne)
Issue Date: 23-Mar-2021
metadata.dc.date: 2021-03-23
Publication information: Archives of Pathology & Laboratory Medicine 2021; online first: 23 March
Abstract: Myocardial fibrosis underpins a number of cardiovascular conditions and is difficult to identify with standard histologic techniques. Challenges include imaging, defining an objective threshold for classifying fibrosis as mild or severe, as well as understanding the molecular basis for these changes. To develop a novel, rapid, label-free approach to accurately measure and quantify the extent of fibrosis in cardiac tissue using infrared spectroscopic imaging. We performed infrared spectroscopic imaging and combined that with advanced machine learning-based algorithms to assess fibrosis in 15 samples from patients belonging to the following 3 classes: (1) nonpathologic (control) donor hearts; (2) patients receiving transplant; and (3) tissue from patients undergoing implantation of ventricular assist device. Our results show excellent sensitivity and accuracy for detecting myocardial fibrosis as demonstrated by high area under the curve of 0.998 in the receiver-operating characteristic curve measured from infrared imaging. Fibrosis of various morphologic subtypes are then demonstrated with virtually generated picrosirius red images, which show good visual and quantitative agreement (correlation coefficient = 0.92, ρ = 7.76 × 10-15) with stained images of the same sections. Underlying molecular composition of the different subtypes were investigated with infrared spectra showing reproducible differences presumably arising from differences in collagen subtypes and/or crosslinking. Infrared imaging can be a powerful tool in studying myocardial fibrosis and gleaning insights into the underlying chemical changes that accompany it. Emerging methods suggest that the proposed approach is compatible with conventional optical microscopy and its consistency makes it translatable to the clinical setting for real-time diagnoses as well as for objective and quantitative research.
URI: https://ahro.austin.org.au/austinjspui/handle/1/26134
DOI: 10.5858/arpa.2020-0635-OA
PubMed URL: 33755723
Type: Journal Article
Appears in Collections:Journal articles

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