Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/19361
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dc.contributor.authorLe, Anh Viet-Phuong-
dc.contributor.authorSzaumkessel, Marcin-
dc.contributor.authorTan, Tuan Zea-
dc.contributor.authorThiery, Jean-Paul-
dc.contributor.authorThompson, Erik W-
dc.contributor.authorDobrovic, Alexander-
dc.date2018-08-28-
dc.date.accessioned2018-09-17T01:47:01Z-
dc.date.available2018-09-17T01:47:01Z-
dc.date.issued2018-08-28-
dc.identifier.citationInternational journal of molecular sciences 2018; 19(9): E2553-
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/19361-
dc.description.abstract(1) Background: Epithelial⁻mesenchymal plasticity (EMP) is a dynamic process whereby epithelial carcinoma cells reversibly acquire morphological and invasive characteristics typical of mesenchymal cells. Identifying the methylation differences between epithelial and mesenchymal states may assist in the identification of optimal DNA methylation biomarkers for the blood-based monitoring of cancer. (2) Methods: Methylation-sensitive high-resolution melting (MS-HRM) was used to examine the promoter methylation status of a panel of established and novel markers in a range of breast cancer cell lines spanning the epithelial⁻mesenchymal spectrum. Pyrosequencing was used to validate the MS-HRM results. (3) Results: VIM, DKK3, and CRABP1 were methylated in the majority of epithelial breast cancer cell lines, while methylation of GRHL2, MIR200C, and CDH1 was restricted to mesenchymal cell lines. Some markers that have been used to assess minimal residual disease such as AKR1B1 and APC methylation proved to be specific for epithelial breast cell lines. However, RASSF1A, RARβ, TWIST1, and SFRP2 methylation was seen in both epithelial and mesenchymal cell lines, supporting their suitability for a multimarker panel. (4) Conclusions: Profiling DNA methylation shows a distinction between epithelial and mesenchymal phenotypes. Understanding how DNA methylation varies between epithelial and mesenchymal phenotypes may lead to more rational selection of methylation-based biomarkers for circulating tumour DNA analysis.-
dc.language.isoeng-
dc.subjectDNA methylation-
dc.subjectbiomarkers-
dc.subjectBreast cancer-
dc.subjectcirculating tumour DNA-
dc.subjectepithelial–mesenchymal plasticity-
dc.subjectmethylation-sensitive high-resolution melting (MS-HRM), pyrosequencing-
dc.subjectminimal residual disease-
dc.titleDNA Methylation Profiling of Breast Cancer Cell Lines along the Epithelial Mesenchymal Spectrum-Implications for the Choice of Circulating Tumour DNA Methylation Markers.-
dc.typeJournal Article-
dc.identifier.journaltitleInternational journal of molecular sciences-
dc.identifier.affiliationSchool of Cancer Medicine, La Trobe University Bundoora, Bundoora, VIC 3086, Australiaen
dc.identifier.affiliationTranslational Research Institute, Woolloongabba, QLD 4102, Australiaen
dc.identifier.affiliationOlivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australiaen
dc.identifier.affiliationDepartment of Clinical Pathology, University of Melbourne, Parkville, VIC 3010, Australiaen
dc.identifier.affiliationCancer Science Institute of Singapore, 14 Medical Drive, National University of Singapore, Singapore 117599, Singaporeen
dc.identifier.affiliationINSERM UMR 1186, Integrative Tumor Immunology and Genetic Oncology, Gustave Roussy, Université Paris-Sud, 94805 Villejuif, Franceen
dc.identifier.affiliationDepartment of Surgery, St. Vincent's Hospital, University of Melbourne, Melbourne, VIC 3065, Australiaen
dc.identifier.affiliationInstitute of Health and Biomedical Innovation and School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, QLD 4059, Australiaen
dc.identifier.doi10.3390/ijms19092553-
dc.identifier.orcid0000-0003-3414-112X-
dc.identifier.pubmedid30154364-
dc.type.austinJournal Article-
local.name.researcherDobrovic, Alexander
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
crisitem.author.deptSurgery (University of Melbourne)-
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