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Title: Advantages and Challenges of Dried Blood Spot Analysis by Mass Spectrometry Across the Total Testing Process.
Austin Authors: Zakaria, Rosita;Allen, Katrina J;Koplin, Jennifer J;Roche, Peter;Greaves, Ronda F
Affiliation: School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, Australia
Clinical Biochemistry, Austin Pathology, Heidelberg, Victoria, Australia
Murdoch Children's Research Institute, Parkville, Victoria, Australia
Department of Paediatrics, University of Melbourne, Parkville, Victoria, Australia
Department of Allergy and Clinical Immunology, Royal Children's Hospital, Parkville, Victoria, Australia
Issue Date: 1-Dec-2016 2016-12
Publication information: EJIFCC 2016; 27(4): 288-317
Abstract: Through the introduction of advanced analytical techniques and improved throughput, the scope of dried blood spot testing utilising mass spectrometric methods, has broadly expanded. Clinicians and researchers have become very enthusiastic about the potential applications of dried blood spot based mass spectrometric applications. Analysts on the other hand face challenges of sensitivity, reproducibility and overall accuracy of dried blood spot quantification. In this review, we aim to bring together these two facets to discuss the advantages and current challenges of non-newborn screening applications of dried blood spot quantification by mass spectrometry. To address these aims we performed a key word search of the PubMed and MEDLINE online databases in conjunction with individual manual searches to gather information. Keywords for the initial search included; "blood spot" and "mass spectrometry"; while excluding "newborn"; and "neonate". In addition, databases were restricted to English language and human specific. There was no time period limit applied. As a result of these selection criteria, 194 references were identified for review. For presentation, this information is divided into: 1) clinical applications; and 2) analytical considerations across the total testing process; being pre-analytical, analytical and post-analytical considerations. DBS analysis using MS applications is now broadly applied, with drug monitoring for both therapeutic and toxicological analysis being the most extensively reported. Several parameters can affect the accuracy of DBS measurement and further bridge experiments are required to develop adjustment rules for comparability between dried blood spot measures and the equivalent serum/plasma values. Likewise, the establishment of independent reference intervals for dried blood spot sample matrix is required.
PubMed URL: 28149263
ISSN: 1650-3414
Type: Journal Article
Subjects: dried blood spots
mass spectrometry
total testing process
Appears in Collections:Journal articles

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