Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/16530
Title: Mammographically dense human breast tissue stimulates MCF10DCIS.com progression to invasive lesions and metastasis
Austin Authors: Huo, Cecilia W;Waltham, Mark;Khoo, Christine;Fox, Stephen B;Hill, Prue;Chen, Shou;Chew, Grace L;Price, John T;Nguyen, Chau H;Williams, Elizabeth D;Henderson, Michael;Thompson, Erik W;Britt, Kara L
Affiliation: Austin Health, Heidelberg, Victoria, Australia
Department of Surgery, University of Melbourne, St Vincent's Hospital, Melbourne, Victoria, Australia
St Vincent's Institute of Medical Research, Melbourne, Victoria, Australia
Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
Department of Pathology, University of Melbourne, Parkville, Victoria, Australia
Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria, Australia
Department of Pathology, St Vincent's Hospital, Melbourne, Victoria, Australia
Northern Health, Epping, Victoria, Australia
College of Health and Biomedicine, Victoria University, St Albans, Victoria, Australia
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria, Australia
Australian Institute for Musculoskeletal Science (AIMSS), Victoria University, University of Melbourne and Western Health, Sunshine Hospital, St Albans, Victoria, Australia
Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia
Translational Research Institute, Woolloongabba, Queensland, Australia
Australian Prostate Cancer Centre - Queensland, Brisbane, Queensland, Australia
Division of Surgery, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia
Metastasis Research Laboratory, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
Issue Date: 25-Oct-2016
Publication information: Breast Cancer Research 2016; 18(1): 106
Abstract: BACKGROUND: High mammographic density (HMD) not only confers a significantly increased risk of breast cancer (BC) but also is associated with BCs of more advanced stages. However, it is unclear whether BC progression and metastasis are stimulated by HMD. We investigated whether patient-derived HMD breast tissue could stimulate the progression of MCF10DCIS.com cells compared with patient-matched low mammographic density (LMD) tissue. METHODS: Sterile breast specimens were obtained immediately after prophylactic mastectomy from high-risk women (nā€‰=ā€‰10). HMD and LMD regions of each specimen were resected under radiological guidance. Human MCF10DCIS.com cells, a model of ductal carcinoma in situ (DCIS), were implanted into silicone biochambers in the groins of severe combined immunodeficiency mice, either alone or with matched LMD or HMD tissue (1:1), and maintained for 6 weeks. We assessed biochamber weight as a measure of primary tumour growth, histological grade of the biochamber material, circulating tumour cells and metastatic burden by luciferase and histology. All statistical tests were two-sided. RESULTS: HMD breast tissue led to increased primary tumour take, increased biochamber weight and increased proportions of high-grade DCIS and grade 3 invasive BCs compared with LMD. This correlated with an increased metastatic burden in the mice co-implanted with HMD tissue. CONCLUSIONS: Our study is the first to explore the direct effect of HMD and LMD human breast tissue on the progression and dissemination of BC cells in vivo. The results suggest that HMD status should be a consideration in decision-making for management of patients with DCIS lesions.
URI: http://ahro.austin.org.au/austinjspui/handle/1/16530
DOI: 10.1186/s13058-016-0767-4
PubMed URL: https://pubmed.ncbi.nlm.nih.gov/27776557
Type: Journal Article
Subjects: Breast cancer
MCF10DCIS.com
Mammographic density
Murine biochamber
Appears in Collections:Journal articles

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