Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13639
Full metadata record
DC FieldValueLanguage
dc.contributor.authorShen, Pei-Juanen
dc.contributor.authorGundlach, Andrew Len
dc.date.accessioned2015-05-16T03:31:18Z
dc.date.available2015-05-16T03:31:18Z
dc.date.issued1998-12-01en
dc.identifier.citationExperimental Neurology; 154(2): 612-27en
dc.identifier.govdoc9878196en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/13639en
dc.description.abstractNoradrenaline, an important transmitter in the CNS, is involved in cerebral plasticity and functional recovery after injury. Experimental brain injury, including KCl application onto the brain surface, induces a slow-moving cortical depolarization/depression wave called cortical spreading depression (CSD). Interestingly, CSD does not produce neuronal damage but can protect cortical neurons against subsequent neurotoxic insults, although the mechanisms involved are unknown. This study examined the status of alpha- and beta-adrenoceptors (ARs) in cerebral cortex following CSD. Anesthetized rats had unilateral CSD induced by a 10-min topical application of KCl to the frontoparietal cortex and were killed at various times thereafter. Levels of alpha1-, alpha2-, beta1-, and beta2-AR mRNA and binding were examined using in situ hybridization histochemistry and radioligand autoradiography. Levels of alpha1b-AR mRNA in the affected neocortex were significantly increased by 20-40% at 1, 2, and 7 days (P </= 0.01) compared with contralateral levels, but were not significantly above control values at 2 and 4 weeks after CSD induction. Cortical alpha1B-AR binding sites were also increased by 45-65% 1 and 2 weeks (P </= 0.01) after CSD in a similar, but delayed, profile to alpha1b-AR mRNA. CSD rapidly increased beta1-AR mRNA by 45% at 1 h (P </= 0.01) and produced a delayed decrease of 25% in alpha2a-AR mRNA at 2 days and 1 week (P </= 0.05), but had no effect on corresponding levels of binding sites. In contrast, CSD had no effect on the remaining AR-subtype mRNAs or binding levels in neocortex under identical conditions. These results reveal a long-term up-regulation of alpha1B-ARs induced by an acute cortical stimulation/depression. Subtype-selective responses of ARs to CSD reflect an important differential regulation of expression of each receptor in vivo and suggest that alpha1B-ARs are particularly likely to be involved in cortical adaptive responses to physical injury at both local and distant locations.en
dc.language.isoenen
dc.subject.otherAction Potentials.drug effectsen
dc.subject.otherAdrenergic alpha-Antagonists.metabolism.pharmacologyen
dc.subject.otherAnimalsen
dc.subject.otherAutoradiographyen
dc.subject.otherBinding, Competitive.physiologyen
dc.subject.otherBrain Chemistry.drug effects.physiologyen
dc.subject.otherCerebral Cortex.chemistry.cytology.physiologyen
dc.subject.otherCortical Spreading Depression.physiologyen
dc.subject.otherElectrophysiologyen
dc.subject.otherGene Expression.physiologyen
dc.subject.otherIn Situ Hybridizationen
dc.subject.otherMaleen
dc.subject.otherNeurons.chemistry.physiologyen
dc.subject.otherNorepinephrine.physiologyen
dc.subject.otherOligonucleotide Probesen
dc.subject.otherPotassium Chloride.pharmacologyen
dc.subject.otherPrazosin.metabolism.pharmacologyen
dc.subject.otherRNA, Messenger.analysisen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.subject.otherReceptors, Adrenergic, alpha-1.genetics.metabolismen
dc.subject.otherSympathomimetics.metabolismen
dc.subject.otherTime Factorsen
dc.subject.otherTritium.diagnostic useen
dc.subject.otherUp-Regulation.physiologyen
dc.titleDifferential spatiotemporal alterations in adrenoceptor mRNAs and binding sites in cerebral cortex following spreading depression: selective and prolonged up-regulation of alpha1B-adrenoceptors.en
dc.typeJournal Articleen
dc.identifier.journaltitleExperimental neurologyen
dc.identifier.affiliationThe University of Melbourne, Department of Medicine, Austin and Repatriation Medical Centre, Heidelberg, Victoria, 3084, Australiaen
dc.identifier.doi10.1006/exnr.1998.6915en
dc.description.pages612-27en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/9878196en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

2
checked on Feb 5, 2023

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.