Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13625
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dc.contributor.authorArabia, A Men
dc.contributor.authorShen, Pei-Juanen
dc.contributor.authorGundlach, Andrew Len
dc.date.accessioned2015-05-16T03:30:55Z
dc.date.available2015-05-16T03:30:55Z
dc.date.issued1998-10-30en
dc.identifier.citationBrain Research. Molecular Brain Research; 61(1-2): 195-202en
dc.identifier.govdoc9795215en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13625en
dc.description.abstractCortical Spreading Depression (CSD) is a slowly propagating wave of depolarization and negative interstitial DC potential, that when induced in the rat brain extends across the entire homolateral hemisphere. Despite evidence that CSD does not penetrate into subcortical regions, neurochemical changes in areas anatomically connected to cortex have been reported. In this study in situ hybridization histochemistry was used to examine the levels of cholecystokinin (CCK), proenkephalin (ENK) and prodynorphin (DYN) mRNA in cortex and forebrain basal ganglia following KCl-induced CSD. Unilateral CSD was induced by topical application of 3 M KCl ( approximately 10 microliter) onto the right parietal cortex for 10 min and rats were then killed 1-6 h and 1-28 days later. CCK mRNA levels were increased (P<0.01) in the ipsilateral neocortex 3 h after CSD (13% above levels in contralateral side), reached a peak at 2 days ( approximately 70%) and were still elevated at 7 (30%) but not, 14 or 28 days later. Unilateral CSD also produced a rapid and sustained increase (P<0.05) in ENK mRNA in ipsilateral piriform cortex (from 3 h to 2 days; 70-250% above contralateral), and a delayed increase in caudate putamen and olfactory tubercle at 1 and 2 days ( approximately 25% in both regions), but levels were again equivalent to control at 7 days and beyond. In contrast, no marked changes in neocortical ENK mRNA, or DYN mRNA in both cortex and basal ganglia, were observed under these conditions. These findings demonstrate that CSD has specific, rapid and long-lasting effects on neuropeptide expression in neocortex and subcortical areas. CSD-induced changes in mesostriatal ENK mRNA are proposed to reflect synaptic activation of local neurons via cortical afferent projections.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherBrainen
dc.subject.otherCerebral Cortex.chemistry.drug effects.metabolismen
dc.subject.otherCholecystokinin.analysis.geneticsen
dc.subject.otherCorpus Striatum.chemistry.drug effects.metabolismen
dc.subject.otherDepression, Chemicalen
dc.subject.otherEnkephalins.analysis.geneticsen
dc.subject.otherGene Expression Regulation.drug effectsen
dc.subject.otherIn Situ Hybridizationen
dc.subject.otherMaleen
dc.subject.otherMembrane Potentials.drug effectsen
dc.subject.otherNeural Pathways.drug effects.physiologyen
dc.subject.otherPotassium Chloride.administration & dosage.pharmacologyen
dc.subject.otherProsencephalon.chemistry.drug effects.metabolismen
dc.subject.otherProtein Precursors.analysis.geneticsen
dc.subject.otherRNA, Messenger.analysis.biosynthesis.drug effectsen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.titleIncreased striatal proenkephalin mRNA subsequent to production of spreading depression in rat cerebral cortex: activation of corticostriatal pathways?en
dc.typeJournal Articleen
dc.identifier.journaltitleBrain Research. Molecular Brain Researchen
dc.identifier.affiliationThe University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin and Repatriation Medical Centre, Heidelberg, Victoria, 3084, Australiaen
dc.description.pages195-202en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/9795215en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
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