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Title: In vitro characterization of a recombinant 32P-phosphorylated anti-(carcinoembryonic antigen) single-chain antibody.
Austin Authors: Patrick, M R;Chester, K A;Pietersz, Geoffrey A
Affiliation: Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 1-Jun-1998
Publication information: Cancer Immunology, Immunotherapy : Cii; 46(4): 229-37
Abstract: The major limitations of monoclonal antibody conjugates as therapeutic agents have been their poor tumour targeting, inadequate tumour penetration and immunogenicity. More even and deeper tissue penetration has been demonstrated with smaller antibody fragments. The smaller size and absence of an Fc segment may contribute to a lowered immunogenicity with single-chain antibodies (scFv) and also permit their recombinant engineering and bacterial expression. We describe the successful engineering, expression and pre-clinical characterisation of a phosphorylatable "kemptide" (Leu-Arg-Arg-Ala-Ser-Gly) anti-carcinoembryonic antigen (anti-CEA) scFv (PKS-scFv), for use as a radioimmunotherapeutic agent. Specifically, a yield of 6 mg/l induced culture was obtained. Site-specific phosphorylation was demonstrated without loss of specificity. In vitro assays revealed a selective cytotoxicity of 32P-PKS-scFv for high-CEA-expressing LS-174T cells compared to the low-CEA-expressing HT-29 cells, with a rapid internalisation rate.
Gov't Doc #: 9671146
Type: Journal Article
Subjects: Binding Sites
Carcinoembryonic Antigen.chemistry.immunology.metabolism
Cloning, Molecular
Colonic Neoplasms.metabolism.radiotherapy
Drug Stability
Escherichia coli.genetics.metabolism
Genetic Engineering.methods
Immunoglobulin Fragments.biosynthesis.chemistry.genetics
Immunoglobulin Variable Region.biosynthesis.chemistry.genetics
Phosphorus Radioisotopes.chemistry
Recombinant Proteins.biosynthesis.chemistry.genetics
Tumor Cells, Cultured
Appears in Collections:Journal articles

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