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dc.contributor.authorUemura, Sen
dc.contributor.authorPompolo, Sen
dc.contributor.authorFurness, J Ben
dc.contributor.authorHardy, Kenneth Johnen
dc.identifier.citationJournal of Gastroenterology and Hepatology; 12(3): 257-65en
dc.description.abstractThe distributions of nerve cells and fibres that are immunoreactive for nitric oxide synthase (NOS) have been investigated in the human gall-bladder. In addition, the colocalization of NOS immunoreactivity (IR) with neuropeptide Y (NPY), pituitary adenylyl cyclase activating peptide (PACAP), somatostatin (SOM), substance P (SP), tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP)-IR was determined. Nitric oxide synthase-IR nerve cell bodies comprised 13 and 30% of nerve cells in ganglia of the fibromuscular and subepithelial layers, respectively. To determine these percentages, neuron-specific enolase-IR was used as a marker for all nerve cells. Although SOM- and VIP-IR nerve cell bodies were found in both ganglia, they rarely contained NOS-IR. In the fibromuscular layer, NOS-IR nerve fibres were abundant and most PACAP-, SOM- and VIP-IR fibres and many NPY-IR fibres were also NOS positive. No colocalization was observed between NOS- and SP- or TH-IR. In the mucosal layer, moderate numbers of NOS-IR fibres were found and the degree of colocalization of NOS-IR with each of NPY-, PACAP-, SOM-, SP- and VIP-IR were as follows: PACAP and NPY > VIP > SOM and SP. Nitric oxide synthase and TH were not colocalized in mucosal fibres. These results suggest that nerve fibres in the fibromuscular layer in the human gall-bladder with the chemical coding NOS/NPY/PACAP/SOM/VIP are axons of inhibitory motor neurons. Nitric oxide synthase-IR fibres in the mucosal layer that contained NPY, PACAP, SOM, SP and VIP with various degrees of colocalization probably contribute to the control of epithelial secretion or absorption.en
dc.subject.otherGanglia.anatomy & histologyen
dc.subject.otherNerve Fibers.ultrastructureen
dc.subject.otherNitric Oxide Synthase.metabolismen
dc.subject.otherPhosphopyruvate Hydratase.metabolismen
dc.subject.otherTissue Distributionen
dc.titleNitric oxide synthase in neurons of the human gall-bladder and its colocalization with neuropeptides.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Gastroenterology and Hepatologyen
dc.identifier.affiliationDepartment of Anatomy and Cell Biology, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.type.austinJournal Articleen
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
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