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Title: Molecular analysis in the diagnosis of pediatric lymphomas.
Austin Authors: Armes, J E;Southey, M;Eades, S;Sturrock, S;McDonald, D;Ellis, D;Chow, C W;Venter, D J
Affiliation: Department of Anatomical pathology, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 6-May-1996
Publication information: Pediatric Pathology & Laboratory Medicine : Journal of the Society For Pediatric Pathology, Affiliated With the International Paediatric Pathology Association; 16(3): 435-49
Abstract: Thirty-five pediatric lymphomas were categorized as either Burkitt's lymphoma (BL), lymphoblastic lymphoma (LL), or large cell anaplastic lymphoma (LCAL) by histological and immunophenotypic methods. They were further characterized by molecular analysis of their antigen receptor genes. Southern blot (SB) and polymerase chain reaction (PCR) techniques were compared in the detection of immunogloblin heavy chain gene (IgH) rearrangement. T cell receptor beta (TCR beta) rearrangements were analyzed by SB and TCR gamma gene rearrangements by PCR. The PCR method of IgH and TCR gamma gene analysis was preferred to the SB methods, because there were fewer equivocal results in IgH gene analysis, TCR gamma rearrangement was more frequently detected than TCR beta in both lymphoblastic and large cell anaplastic lymphomas, and the PCR technique was more rapid, required less DNA, and could be used with archival material. In addition, analysis of IgH gene rearrangement by PCR was more specific for assessing B cell lineage. Although most of the molecular data were easily interpreted, occasional ambiguous results were seen due to genetic events other than antigen receptor gene rearrangement affecting the genetic analysis. Thus, it is imperative to interpret these genetic data in the context of adequate morphological and immunophenotypic analysis.
Gov't Doc #: 9025844
Journal: Pediatric pathology & laboratory medicine : journal of the Society for Pediatric Pathology, affiliated with the International Paediatric Pathology Association
Type: Journal Article
Subjects: Blotting, Southern
Burkitt Lymphoma.diagnosis.genetics.immunology
Child, Preschool
Gene Rearrangement, B-Lymphocyte.genetics
Gene Rearrangement, T-Lymphocyte.genetics
Genes, Immunoglobulin.genetics
Lymphoma, Large B-Cell, Diffuse.diagnosis.genetics.immunology
Molecular Biology.methods
Polymerase Chain Reaction
Precursor Cell Lymphoblastic Leukemia-Lymphoma.diagnosis.genetics.immunology
Receptors, Antigen, T-Cell, alpha-beta.genetics
Receptors, Antigen, T-Cell, gamma-delta.genetics
Appears in Collections:Journal articles

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