Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13466
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dc.contributor.authorO'Shea, R Den
dc.contributor.authorGundlach, Andrew Len
dc.date.accessioned2015-05-16T03:19:16Z
dc.date.available2015-05-16T03:19:16Z
dc.date.issued1996-06-01en
dc.identifier.citationJournal of Neuroendocrinology; 8(6): 417-25en
dc.identifier.govdoc8809671en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/13466en
dc.description.abstractNitric oxide (NO) is produced by the enzyme NO synthase (NOS) and may be involved in the regulation of nutrient and endocrine homeostasis via actions on neurones of the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei. The effects of water deprivation or food deprivation for 4 days on the abundance of messenger RNA encoding NOS in these nuclei in rats were examined using in situ hybridization. Water deprivation markedly increased the abundance of NOS mRNA in both the SON and PVN (225 +/- 11% of control, P < 0.05 and 261 +/- 34% of control, P < 0.01 respectively). NOS mRNA abundance also appeared to be increased in magnocellular accessory nuclei. Food deprivation decreased NOS mRNA abundance in the SON and PVN (42 +/- 6% and 52 +/- 7% of control respectively, both P < 0.05), while withdrawal of both food and water produced no significant net changes in the abundance of NOS mRNA. Treatment-induced alterations in NOS mRNA abundance were reflected by changes in NOS activity, as assessed by NADPH-diaphorase histochemistry, and NADPH-diaphorase staining was observed in neurones both positive and negative for oxytocin-like immunoreactivity. These findings suggest that NOS mRNA abundance, NOS enzymatic activity and presumably NO production are modulated in an activity-dependent manner in hypothalamic (magnocellular and parvocellular) neurones by alterations in fluid and nutrient homeostasis, and support data from other studies suggesting a role for NO in the central regulation of water and food intake in the rat.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherFood Deprivationen
dc.subject.otherGene Expressionen
dc.subject.otherHypothalamus.enzymology.physiologyen
dc.subject.otherImmunohistochemistryen
dc.subject.otherIn Situ Hybridizationen
dc.subject.otherMaleen
dc.subject.otherNADPH Dehydrogenase.analysisen
dc.subject.otherNeurons.enzymologyen
dc.subject.otherNeurosecretionen
dc.subject.otherNitric Oxide Synthase.genetics.metabolismen
dc.subject.otherOxytocin.analysisen
dc.subject.otherParaventricular Hypothalamic Nucleus.chemistry.enzymologyen
dc.subject.otherRNA, Messenger.metabolismen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.subject.otherSupraoptic Nucleus.chemistry.enzymologyen
dc.subject.otherWater Deprivationen
dc.titleFood or water deprivation modulate nitric oxide synthase (NOS) activity and gene expression in rat hypothalamic neurones: correlation with neurosecretory activity?en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of neuroendocrinologyen
dc.identifier.affiliationUniversity of Melbourne Clinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin and Repatriation Medical Centre, Victoria, Australiaen
dc.description.pages417-25en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8809671en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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