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dc.contributor.authorDean, Rachael Gen
dc.contributor.authorZhuo, Jen
dc.contributor.authorAlcorn, Den
dc.contributor.authorCasley, David Jen
dc.contributor.authorMendelsohn, Frederick AOen
dc.identifier.citationClinical and Experimental Pharmacology & Physiology; 23(6-7): 524-31en
dc.description.abstract1. We have previously shown that [125I]-endothelin (ET) receptor binding is localized almost exclusively to the fenestrated endothelial cells of glomerular capillaries and peritubular capillaries in the rat kidney following systemic administration of the radioligand in vivo. Because of the lack of specific ET receptor binding in other glomerular and tubular structures following in vivo labelling, we undertook further studies, using electron microscopic autoradiography and ET receptor subtype selective ligands, to investigate whether other renal components also contain ET receptor binding and, if so, to determine the cellular localization of the ET receptor subtypes, ETA and ETB, following in vitro labelling. 2. At the electron microscopic level, ET binding sites were localized primarily to the fenestrated endothelium of glomerular and peritubular capillaries of the cortex, inner stripe of the outer medulla and the inner medulla. ET binding sites also occurred overlying renomedullary interstitial cells (RMIC) of the inner medulla. 3. The ETB receptor selective agonist, sarafotoxin 6c (S6c), abolished ET binding in the vascular endothelium throughout the kidney, while the ETA receptor selective antagonist, BQ123, was without effect. Both BQ123 and S6c partially inhibited the binding in the RMIC of the inner medulla. 4. These results indicate that ET receptor binding in the fenestrated endothelium in the glomerular capillaries and peritubular capillaries belongs mainly to the ETB subtype, whereas both ETA and ETB subtypes are present in the RMIC.en
dc.subject.otherCapillaries.drug effects.metabolismen
dc.subject.otherEndothelin Receptor Antagonistsen
dc.subject.otherEndothelium, Vascular.drug effects.metabolismen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherIodine Radioisotopes.diagnostic useen
dc.subject.otherMicroscopy, Electronen
dc.subject.otherPeptides, Cyclic.diagnostic useen
dc.subject.otherRats, Sprague-Dawleyen
dc.subject.otherReceptors, Endothelin.agonists.metabolismen
dc.subject.otherVasoconstrictor Agents.diagnostic useen
dc.subject.otherViper Venoms.diagnostic useen
dc.titleCellular localization of endothelin receptor subtypes in the rat kidney following in vitro labelling.en
dc.typeJournal Articleen
dc.identifier.journaltitleClinical and Experimental Pharmacology & Physiologyen
dc.identifier.affiliationUniversity of Melbourne, Department of Medicine, Austin, Australiaen
dc.type.austinJournal Articleen
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
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