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Title: Cellular localization of endothelin receptor subtypes in the rat kidney following in vitro labelling.
Austin Authors: Dean, Rachael G;Zhuo, J;Alcorn, D;Casley, David J;Mendelsohn, Frederick AO
Affiliation: University of Melbourne, Department of Medicine, Austin, Australia
Issue Date: 7-Jun-1996
Publication information: Clinical and Experimental Pharmacology & Physiology; 23(6-7): 524-31
Abstract: 1. We have previously shown that [125I]-endothelin (ET) receptor binding is localized almost exclusively to the fenestrated endothelial cells of glomerular capillaries and peritubular capillaries in the rat kidney following systemic administration of the radioligand in vivo. Because of the lack of specific ET receptor binding in other glomerular and tubular structures following in vivo labelling, we undertook further studies, using electron microscopic autoradiography and ET receptor subtype selective ligands, to investigate whether other renal components also contain ET receptor binding and, if so, to determine the cellular localization of the ET receptor subtypes, ETA and ETB, following in vitro labelling. 2. At the electron microscopic level, ET binding sites were localized primarily to the fenestrated endothelium of glomerular and peritubular capillaries of the cortex, inner stripe of the outer medulla and the inner medulla. ET binding sites also occurred overlying renomedullary interstitial cells (RMIC) of the inner medulla. 3. The ETB receptor selective agonist, sarafotoxin 6c (S6c), abolished ET binding in the vascular endothelium throughout the kidney, while the ETA receptor selective antagonist, BQ123, was without effect. Both BQ123 and S6c partially inhibited the binding in the RMIC of the inner medulla. 4. These results indicate that ET receptor binding in the fenestrated endothelium in the glomerular capillaries and peritubular capillaries belongs mainly to the ETB subtype, whereas both ETA and ETB subtypes are present in the RMIC.
Gov't Doc #: 8800578
Journal: Clinical and Experimental Pharmacology & Physiology
Type: Journal Article
Subjects: Animals
Capillaries.drug effects.metabolism
Endothelin Receptor Antagonists
Endothelium, Vascular.drug effects.metabolism
In Vitro Techniques
Iodine Radioisotopes.diagnostic use
Microscopy, Electron
Peptides, Cyclic.diagnostic use
Rats, Sprague-Dawley
Receptors, Endothelin.agonists.metabolism
Vasoconstrictor Agents.diagnostic use
Viper Venoms.diagnostic use
Appears in Collections:Journal articles

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