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Title: Rapid but transient increases in cholecystokinin mRNA levels in cerebral cortex following amygdaloid-kindled seizures in the rat.
Austin Authors: Burazin, T C;Gundlach, Andrew L
Affiliation: University of Melbourne, Department of Medicine, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia
Issue Date: 3-May-1996
Publication information: Neuroscience Letters; 209(1): 65-8
Abstract: Cholecystokinin-octapeptide (CCK-8S) is widely distributed in neurones of the central nervous system, where it is thought to act as a transmitter or modulator. CCK-8S has been shown to exert anti-convulsant activity in animal seizure models and changes in cortical and hippocampal CCK-immunoreactivity and preproCCK messenger RNA (mRNA) have been reported following electrically- and chemically-induced seizures. In the present study, the spatiotemporal effect of amygdaloid-kindled seizures on levels of preproCCK messenger RNA in rat brain were determined using quantitative in situ hybridization histochemistry. Stimulation-evoked seizures produced bilateral increases (45-70%) in preproCCK mRNA throughout layers II-III of the cerebral cortex. These increases were rapidly induced, occurring 30-60 min after the last stage 5 seizure, but transient, as no significant changes were detected after 2 h, or subsequently at 24 or 72 h, or 2-8 weeks, post-stimulation. Rapid changes in the relative levels of preproCCK mRNA, post-seizure, suggest a possible stabilization of preproCCK transcripts and increased production of CCK-8S peptide, which may be involved in anticonvulsant mechanisms in response to the acute seizures.
Gov't Doc #: 8734911
Journal: Neuroscience letters
Type: Journal Article
Subjects: Amygdala.metabolism
Cerebral Cortex.metabolism
Kindling, Neurologic
Protein Precursors.biosynthesis
RNA, Messenger.biosynthesis
Rats, Sprague-Dawley
Sincalide.analogs & derivatives.biosynthesis
Time Factors
Transcription, Genetic
Appears in Collections:Journal articles

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