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Title: Extracellular mutations of non-obese diabetic mouse FcgammaRI modify surface expression and ligand binding.
Austin Authors: Gavin, A L;Hamilton, J A;Hogarth, P Mark
Affiliation: Austin Research Institute, Austin Hospital, Heidelberg, Victoria 3084, Australia
Issue Date: 19-Jul-1996
Publication information: The Journal of Biological Chemistry; 271(29): 17091-9
Abstract: The non-obese diabetic mouse (NOD) expresses a unique form of the high affinity receptor for IgG (FcgammaRI), containing multiple mutations that result in substitutions and insertions of amino acids and a truncated cytoplasmic tail. As a result of these major changes, receptor affinity for IgG increases 10-fold over that of wild-type FcgammaRI from BALB/c mice, while the specificity for ligand is retained. Kinetic analysis revealed that while the association rate of IgG with FcgammaRI from NOD mice (FcgammaRI-NOD) and FcgammaRI from BALB/c mice (FcgammaRI-BALB) is similar, IgG bound much more tightly to FcgammaRI-NOD as revealed by a profoundly diminished dissociation rate. Transfection studies demonstrated that FcgammaRI-NOD was expressed at one-tenth of the level of FcgammaRI-BALB. Although mouse FcgammaRI was previously not known to associate with the FcepsilonRI gamma-subunit, transfection of COS-7 cells demonstrates that like human FcgammaRI, mouse FcgammaRI is also able to associate with this signaling subunit. Furthermore, expression levels of FcgammaRI-NOD were not restored by the presence of the FcepsilonRI gamma-subunit. The difference in the levels of expression was mapped to mutations in the extracellular region of FcgammaRI-NOD as replacement of the extracellular domains with those of human FcgammaRI or FcgammaRI-BALB restored expression to that of human FcgammaRI or FcgammaRI-BALB.
Gov't Doc #: 8663283
Type: Journal Article
Subjects: Amino Acid Sequence
Bone Marrow.immunology
Bone Marrow Cells
Cell Line
Cercopithecus aethiops
DNA Primers
DNA, Complementary
Diabetes Mellitus, Type 1.genetics.immunology
Flow Cytometry
Immunoglobulin G.metabolism
Mice, Inbred BALB C
Mice, Inbred NOD
Molecular Sequence Data
Mutagenesis, Site-Directed
Polymerase Chain Reaction
Receptors, IgG.biosynthesis.genetics.metabolism
Recombinant Fusion Proteins.biosynthesis.metabolism
Sequence Homology, Amino Acid
Substrate Specificity
Appears in Collections:Journal articles

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